EFFECT OF AMIDATED ALGINATE ON CHOLESTEROL IN SERUM, LIVER AND FAECES OF RATS FED FAT AND CHOLESTEROL-CONTAINING DIET

Bìol. Tvarin, 2016, volume 18, issue 4, pp. 53–57 http://dx.doi.org/10.15407/animbiol18.04.053

EFFECT OF AMIDATED ALGINATE ON CHOLESTEROL IN SERUM, LIVER AND FAECES OF RATS FED FAT AND CHOLESTEROL-CONTAINING DIET

M. Marounek¹, Z. Volek¹, T. Taubner¹, D. Duškova¹, L. Kalachniuk², D. Tsvilikhovskyi²

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¹Institute of Animal Science,
Přátelství 815, Prague 22, Czech Republic

²National University of Life and Environmental Sciences of Ukraine,
15 Heroiv Oborony str., Kyiv 03041, Ukraine

Polysaccharides (of which there is alginic acid) play a key role in the digestion and absorption of nutrients because they can increase the viscosity and form a gel in the intestine. Considering results of our previous studies of the effect of pectin, cellulose and their amidated derivatives on digestion in rats and experimental data of other scientists, we decided to study the effect of amidated alginate on level of cholesterol in the blood serum, liver and feces in the rats which were fed by diet supplemented with fat and cholesterol.

Female rats were fed a control (1) (basal diet without cholesterol and amidated alginate sumplements) and two experimental (2 and 3) diets supplemented with cholesterol (10 g/kg) and amidated alginate (0 and 40 g/kg, respectively). All diets contained palm fat. In rats fed the cholesterol-containing diet, amidated alginate significantly decreased serum total and low density lipoprotein cholesterol, hepatic cholesterol and lipids in the liver tissue. Amidated alginate increased faecal concentration of fat from 61 to 91 mg/g. In the rats fed amidated alginate, the faecal concentrations of cholesterol, bile acids and total sterols were not significantly influenced. It can be concluded that the reduction of cholesterolemia in treated rats was probably based on the removal of fat from the intestine, rather than on interruption of cholesterol circulation in the liver.

Keywords: RATS, FAECES, CHOLESTEROL, FATS, BILE ACIDS, N-ALKYL AMIDATED ALGINATE

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