EFFICIENCY OF DIAGNOSTICS OF CATTLE TOXOPLASMOSIS WITH THE HELP OF THE IMMUNE BIOSENSOR

Bìol. Tvarin, 2016, volume 18, issue 4, pp. 35–39 http://dx.doi.org/10.15407/animbiol18.04.035

EFFICIENCY OF DIAGNOSTICS OF CATTLE TOXOPLASMOSIS WITH THE HELP OF THE IMMUNE BIOSENSOR

M. V. Halat, K. Ye. Shavanova, M. B. Strilchuk, N. F. Shpyrka, M. F. Starodub

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National University of Life and Environmental Sciences of Ukraine,
15 Heroiv Oborony str., Kyiv 03041, Ukraine

For life-time toxoplasmosis diagnostics a significant number of laboratory methods were offered. However, their effectiveness is not always sufficient to the needs of specialists of veterinary medicine. In this regard a new method of life-time diagnostics of this disease using immune biosensor “Plazmotest” based on surface plasma resonance (SPR) was developed and tested. The study of blood serum of cattle in order to establish the diagnosis of toxoplasmosis was conducted in laboratories of Biosensors and Department of Parasitology and Tropical Veterinary NULES of Ukraine during 2014–2016. In the experiments 48 animals of different cattle breeds hold in different conditions were used. The age of animals was from 5 months to 7 years.

The results of investigations of blood sera with the help of immune biosensor showed a higher effectiveness regarding its detection an agent of parasitic unicellular organism Toxoplasma gondii in the of cattle organism compared to the results obtained by applying the method of enzyme immunoassay (ELISA).

No significant difference of the degree of infection with toxoplasmosis agent in cattle males and females was found. An increase of the infestation intensity with the age of animals was proved. So, at the age of four years, 4.8 % of the animals reacted positively to the presence of antibodies to Toxoplasma, while in cattle older than four years — 29.6 %.

Keywords: TOXOPLASMOSIS, CATTLE, TOXOPLASMA GONDII, SERUMS OF BLOOD, METHODS OF DIAGNOSIS, ELISA, IMMUNE BIOSENSOR

1. Dubey J. P. Toxoplasmosis of animals and humans. 2^nd editionю 2013, 317 p.

2. Dubey J. P., Jones J. L. Toxoplasma gondii infection in humans and animals in the United States. International Journal of Parasitology, 2008, no. 38, pp. 1257–1278. https://doi.org/10.1016/j.ijpara.2008.03.007

3. Dubey J. P., Prowell M. Ante-mortem diagnosis, diarrhea, oocyst shedding, treatment, isolation, and genetic typing of Toxoplasma gondii associated with clinical toxoplasmosis in a naturally infected cat. Journal of Parasitology, 2013, vol. 99 (1), pp. 158–160. https://doi.org/10.1645/GE-3257.1

4. Galat M. V. Toxoplasma gondii — dangerous parasite of ruminants and man. Veterinary Medicine of Ukraine, 2015, no. 7, pp. 25–27. (in Ukrainian)

5. Jiang S., Hua E., Liang M., Liu B., Xie G. A novel immunosensor for detecting Toxoplasma gondii-specific IgM based on goldmag nanoparticles and graphene sheets. Colloids and surfaces. B. Biointerfaces., 2013, no. 101, pp. 481–486. https://doi.org/10.1016/j.colsurfb.2012.07.021

6. Luo Y., Liu X., Jiang T., Liao P., Fu W. Dual-aptamer-based biosensing of toxoplasma antibody. Analytical Chemistry, 2013, no. 85 (17), pp. 8354–8360.

7. Meng K., Sun W., Zhao P., Zhang L., Cai D., Cheng Z., Guo H., Liu J., Yang D., Wang S., Chai T. Development of colloidal gold-based immunochromatographic assay for rapid detection of Mycoplasma suis in porcine plasma. Biosensors Bioelectronics, 2014, no. 55, pp. 396–399. https://doi.org/10.1016/j.bios.2013.12.048

8. Starodub N. F. Biosensors for the Control of Biochemical Parameters in the Diagnostics of Diseases. Book of series in sensors, Portable Biosensing of Food Toxicants and Environmental Pollutants, CRC Press, Taylor & Francis Croup Boca Raton London, New York, 2013, pp. 743–775.

9. Wang H., Lei C., Li J., Wu Z., Shen G., Yu R. A piezoelectric immunoagglutination assay for Toxoplasma gondii antibodies using gold nanoparticles. Biosensors Bioelectronics, 2004, no. 19 (7), pp. 701–709.

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