Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 125–134
MICROELEMENTS CONTENT IN TISSUES OF FEMALE F0 RATS AND F1 MALES AT THE WATERING OF NANO AND CHEMICALLY SYNTHESIZED GERMANIUM CITRATE
M. I. Khrabko1, R. S. Fedoruk1, M. I. Khrabko2, E. E. Martsinko3, H. H. Denys1
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1Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv, 79034, Ukraine
2Vyshnyanskyy College Lviv National Agrarian University,
v. Vyshnyа, Lviv region, 81540, Ukraine
3I. I. Mechnikov Odesa National University,
2 Dvoryanska str., Odesa 65082, Ukraine
The results of the impact of long watering different amounts of citrate germanium nanotechnology and obtained by chemical synthesis, on the content of microelements in tissues of female and male mothers to their offspring are presented in the paper. A credible intergroup gender and age differences content of copper, cobalt, manganese, iron and zinc in tissues of liver, kidneys, lungs and muscles have been established.
In the tissues of female rats in the 2nd experimental group after watering 10 mg Ge, the content of microelements is the highest, as it is indicated by the significant increase of Cu in all organs, Co, Mn and Zn —in kidneys, lungs and muscles of female rats. Increasing of Ge concentrations in the water causes the reduction of Co, Mn and Zn in these organs, which is confirmed by watering chemically synthesized germanium. Increasing doses of citrate Ge 20 and 200 mg influenced the reduction of Co and Mn in all tissues of males in 3rd and 4th groups. Some differences between the yields of microelements in tissues of liver, kidneys, lungs and muscles of males and females in 2nd group receiving 10 mg Ge are typical. For the 3rd and the 4th groups the content of most of the elements kept their tendency in both females and males of these groups, except lower Zn content in kidneys, Fe — in muscles in males of 3rd group, but higher levels of Mn in this tissue and Fe — in lungs and muscles in 4th group and lower Cu in the muscles of these males. Watering chemically synthesized citrate Ge in amount 200 and 2000 mg of Ge led to unidirectional changes in the tissues of the internal organs and muscles of the likely increase in the level of Co and Mn in the liver, lungs and muscles, Cu — in lungs and muscles, Mn — in kidneys and muscles in female rats of 5th group and Zn — in muscles in female rats of the 5th and the 6th,in kidneys in the 6th groups against the backdrop of lower (P<0,01) Cu content in the liver in the 5th, Co and Mn in liver, lungs and muscles, Cu and Co in kidneys in females of the 6th group. The content of microelements in tissues of males of 6th group maintained tendency of significant difference in their mothers compared with control group for most of the tissues and elements, including: liver, besides Zn, kidneys and lungs, in addition to Fe and Zn, and muscles except Cu.
Uneven effects of different doses of citrate Ge, obtained by nanotechnology and chemical synthesis, on the level of the individual elements in the tissues of females and males from their offspring, confirmed the absolute content of trace elements in terms of weight investigated organs for which the same tendency of intergroup differences in most definitions was kept for both mothers F0 and their offspring F1.
Keywords: RATS, GERMANIUM CITRATE, TISSUES, MICROELEMENTS
1. Borisevich V. B., Kaplunenko V. G., Kosinov N. V. Nanomaterials in biology. Fundamentals nanoveterinary. Kyiv, Avicenna, 2010, 416 p. (in Ukrainian)
2. European convention for the protection of vertebrate animals used for experim and other scientific purposes. Coun. of Europe, Strasbourg, 1986, 53 p.
3. Fedoruk R. S., Khrabko M. I., Dolaychuk O. P., Tsap M. M. The growth and development of the F1 rats when watering them, and female-mothers of different doses of germanium citrate. Collection of works of scientific symposium with international participation “Zootechnycal science — an important factor for the European type of the agriculture”, Moldova, Maximovca, 2016, pp. 780–785.
4. Fedoruk R. S., Khrabko M. I., Tsap M. M., Martsynko O. E. Growth, development and reproductive function of female rats and their offspring viability at the conditions of the watering of different doses of citrate germanium. The Animal biology, 2016, vol. 18, no. 3, pp. 97–106. (in Ukrainian)
5. Flyd O. D., Gard T. K., Vernadsky A., Mironov V. F. Connections of pentaco-ordinated Germanium. I. Hermotsyny, hermolany and hermokan. ZHOH, 2008, pp. 2745–2750. (in Russian)
6. Jeyaraman V., Sellappa S. In-vitro anticancer activity of organic germanium on human breast cancer cell line (MCF-7). Journal of Current Pharmaceutical Research, 2011, vol. 5 (1), pp. 39–41.
7. Kresyun V. I., Shemonayeva K. F., Vidavska A. G. Pharmacological characterization of compounds of germanium. Clinical Pharmacy, 2004, no. 4, pp. 65–68. (in Ukrainian)
8. Kudrin A. V., Skalny A. V., Larks A. A., Rocky M. G., Gromova O. A., Germanium and immune response. In the: Immunopharmacology microelements. Moscow, KMC Publ., 2000, 386 p. (in Russian)
9. Law of Ukraine no. 3447-IV “On protection of animals from cruelty”. Supreme Council of Ukraine, Official. kind., 2006, 27, 990: 230. (Library of official publications).
11. Lukevics E. J., Gard T. K., Ignatovich L. M., Mironov V. F. The biological activity of compounds of germanium. Riga, Zinatne, 1990, 191 p. (in Russian)
12. Lukyanchuk V. D., Nemyatyh O. D. Influence of coordination compounds of germanium with nicotinic acid on the activity of enzymes of energy metabolism under extreme oxygen-deficient state. Ukrainian Journal of extreme medicine behalf G. Mozhaeva, 2003, no. 1, pp. 62–66. (in Ukrainian)
13. Melnychuk S. D., Moklyachuk L. I., Draha M. V. Integrated provision of human micronutrients — problems and solutions. Agroecological journal, 2012, no. 2, pp. 24–27. (in Ukrainian)
14. Oberlis D., Garland B., Skalny A. The biological role of macro- and micronutrients in humans and animals. SPb.,The science, 2008, 542 p.
15. Perez Gallardo L., Sanchez-Mayoral A., Miguel R., Insern F., Romera J. A., Granados I., Santos A. Copper and zinc status in different rat tissues after the administration of ginseng. Metal Ions in Biology and Medicine, Paris, 1996, vol. 4, pp. 336–338.
16. Pogorelov M. V., Bumeyster V. I., Tkach G. F. Macro- and microelements (metabolism, pathology and determination methods). A monograph. Sumy, 2010, 147 p. (in Ukrainian)
17. Seba M. V., Losev O. M., Chorniy B. M., Kaplunenko V. G. Survivability bees for food different concentrations carboxylate acids in the composition of sugar syrup. Scientific Bulletin of NULES of Ukraine, Series of “Technology of production and processing of livestock products”, 2015, vol. 223, pp. 167–176. (in Ukrainian)
18. Shafran L. M., Pyhteeva E. G., Bolshoy D. V. Metallothioneins. Ed. by prof. Shafran L. M. Odessa, Chernomorye, 2011, 428 p. (in Russian)
19. Skalny A. V., Rudakov I. A. Bioelements in medicine. “Onyx 21” Publishing House, World, 2004, 272 p. (in Russian)
20. Trachtenberg I. M., Chekman I. S., Linnik V. O., Kaplunenko V. G. Interaction micronutrients, biological, medical and social aspects. Bulletin of the National Academy of Sciences, 2013, vol. 6, pp. 11–20. (in Ukrainian)
21. Ukraine patent for utility model number 38391. IPC (2006): C07C 51/41, C07F 5/00, C07F 15/00, C07C 53/126 (2008.01), C07C 53/10 (2008.01), A23L 1/00, B82B 3/00. Method metal carboxylates “Nanotechnology receiving metal carboxylates”. Kosinov M. V., Kaplunenko V. G. Publish. 12.01.2009, Bull. no. 1. (in Ukrainian)
22. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary. A reference book. Ed. by V. V. Vlizlo. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
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