Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 119–124
DYNAMICS OF PHAGOCYTIC ACTIVITY OF NEUTROPHILS IN RATS UNDER OXIDATIVE STRESS AND THE ACTION OF LIPOSOMAL PREPARATION
M. Khariv, B. Gutyj
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Lviv national university of veterinary medicine and biotechnologies named after S. Z. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine
The article deals with the researches results of the developed complex liposomal preparation influence on dynamics of nonspecific level of natural resistance in rats under modeled oxidative stress caused with the use of carbon tetrachloride.
It is proved that intramuscular injection to rats in the experimental group 50 % of oil solution of tetra chloromethane at a dose of 0.25 ml per 100 g of animal body weight determines the antigenic load on the body and causes nonspecific inhibition level of natural resistance. This is indicated with decrease in phagocytic activity of neutrophilsin the blood of infected animals during the entire research period. On the 14th day the indices of phagocyte activity of neutrophils was 42.6 % against 48.9 % in the control group of animals. Alongside with this reduction of values it was found phagocyte index and phagocyte number of blood among rats on the 2nd, 5th and 10thday of research. On the 14th day of the experiment, these indicators in biological object of rat blood of the 1st experimental group were low: accordingly, the value of phagocyte index by 38 % and phagocyte number — 18 % from indicators of the rat organism by oxidative stress.
It is advisable to apply the liposomal preparation containing butafosfan, interferon, milk thistle and vitamins. After using liposomal preparation to rats, under oxidative stress during researches, equilibrium level of nonspecific natural resistance is restored in the blood, namely no abnormal parameters of phagocyte activity of neutrophils, phagocyte index and phagocyte number of blood. On the 14th day of experiment the indices of nonspecific link of the immune system in the blood of rats varied within the physiological limits.
Keywords: OXIDATIVE STRESS, LIPOSOMAL PREPARATION, PHAGOCYTE ACTIVITY, EUTROPHILS, MILK THISTLE, RATS BODY, IMMUNE SYSTEM, LIPOSOMAL PREPARATION, BUTAFOSFAN, INTERFERON, MILK THISTLE, VITAMINS
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