EFFECT OF 5-DAY EXPOSURE OF VITAMIN A AND E ON STATUS OF RED BLOOD CELL ANTIOXIDANT SYSTEM AND HEMATOLOGICAL PARAMETERS OF RATS INTOXICATED BY CHLORPYRIFOS

Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 106–114

http://doi.org/10.15407/animbiol19.02.106

EFFECT OF 5-DAY EXPOSURE OF VITAMIN A AND E ON STATUS OF RED BLOOD CELL ANTIOXIDANT SYSTEM AND HEMATOLOGICAL PARAMETERS OF RATS INTOXICATED BY CHLORPYRIFOS

V. P. Rosalovsky

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Institute of Animal Biology NAAS,
38 Stus str., Lviv 79034, Ukraine

The paper is dedicated to the investigation of effects of a single oral administration of chlorpyrifos, a common organophosphate pesticide, at a dose of 90 mg/kg, on the number of blood cells in peripheral blood, red blood cells resistance, oxygen-transport function of hemoglobin, and antioxidant protection indicators of red blood cells in rats in conditions of oral administration of a mixture of vitamin A and E for 5 days.

We observed that exposure to 90 mg/kg CPF led to a decrease in the total count of red blood cells, platelets, with hemoglobin content, and to increased number of leucocytes. Moreover, we observed a decrease in erythrocytes resistance to acid hemolysis and hemoglobin affinity for oxygen. In the antioxidant defense system, we found increased activity of SOD and CAT in erythrocytes, TBARS and LPO content. It was researched that 90 mg/kg CPF caused a decrease in GST and GPO activity and content of GSH.

We found that daily oral administration of vitamins A and E (at doses of 10000 IU and 0.1 g, respectively) for 5 days simultaneously with CPF led to normalization of erythrocyte quantity and hemoglobin content, increased red blood cells resistance, activity of GST and GPO decrease of TBARS, content, CAT activity, increased and normalization of GSH content. Under conditions of vitamin administration, we observed increase in the number of blood cells (red blood cells, and platelets), and in erythrocytes resistance to acid hemolysis. At the same time, the vitamin mixture caused normalization of prooxidant-antioxidant balance that may indicate a protective effect of used doses of vitamins.

Keywords: CHLORPYRIFOS, VITAMIN E, VITAMIN A, RED BLOOD CELLS, BLOOD, ANTIOXIDANT SYSTEM, RATS

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