Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 50–55
BLOOD PLASMA PROTEIN OF CARP UNDER THE INFLUENCE OF SULFANILAMIDE
M. Kurbatova
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National University of Life and Environmental Sciences of Ukraine,
11 Geroiv Oborony str., Kyiv 03041, Ukraine
The protein spectrum of the blood plasma of carp doublets according to the data of PAGE is characterized by the presence of 17 fractions of proteins located in the zones of prealbumins, albumins, transferrins, ceruloplasmins, haptoglobins, fibrinogens, glycoproteins and lipoproteins. The molecular weight of the proteins of the detected fractions ranges from 25 to 450 kD and more, and their number in the plasma of the fish and the fractional composition depended on the content of sulfonamide in the aquarium water. When the concentration of sulfonamides in water was 0.015 mg/dm3, the total content and fractional composition of the plasma proteins of fish compared to the control did not change, except for proteins with a molecular weight 260 kDa, whose level decreased by 28 %.
The increase in the concentration of sulfanilamide in the aquarium water to 0.15 mg/dm3 influenced the fractional composition of carp blood plasma proteins to a greater extent, as evidenced by the appearance on the forogram of four new protein bands with a molecular mass of more than 140 kDa (zone F), about 100 kDa (zone H and J) and less than 50 kDa (zone P), as well as the absence of proteins in the L zones (more than 60 kDa), O (50 kDa) and Q (35 kDa). In addition, an increase in 76 % of the protein content with a molecular weight of 60 kDa and a decrease in the level of proteins with a molecular weight 260 kD or less (C and D fractions), respectively, by 35 % and 48 % in the blood plasma of fish with a sulfanilamide concentration 0.15 mg/dm3 in water compared to the control was registered.
In fish with a concentration of sulfonamide 0.30 mg/dm3 in the aquarium water the effect of the mentioned xenobiotic on the fractional composition of blood plasma proteins was manifested in a decrease of the level of proteins of fractions C and D (260 kDa and less), G (140 kDa) and an increase in the protein content of the M fraction (60 kDa), in parallel with the appearance of a number of new fractions (F, H, J and P), which may be a consequence of a change in the electrophoretic mobility of proteins.
Sulfonamid added to the aquarium water at a concentration of 0.015, 0.15 and 0.30 mg/dm3 had no effect on the total protein content in fish blood plasma.
Keywords: CARP, BLOOD PLASMA, PROTEINS, SULFANILAMIDE
1. Baklashova T. A. Workshop on ichthyology. Moscow, Agropromizdat, 1990, 223 p. (in Russian)
2. Bukreeva E. M., Lositsky O. S., Zimin Y. V. The biological activity of some derivatives of sulfanilamide and diaminopyrimidina. Modern problems of science and education, 2011, no. 3, (in Russian)
3. Chen J., Zhou X., Zhang Y., Gao H. Potential toxicity of sulfanilamide antibiotic: binding of sulfamathazine to human serum albumin. Sci. Totae Environ., 2012, tug. 15, vol. 432, pp. 269–274. DOI: 10.1016/J.scintotenv. 2012. 06.003. Epub. 2012. jun.27.
4. Colman J., Rem K.-H. Visual Biochemistry. M., March, 2004, 469 p.
5. Gornely S. Determination of serum protein by mean of biuret reaction. Journal of Biochemistry, 1949, vol. 177, no. 2, pp. 751–755.
6. Gulkova A., Leung H. W., Jamashita N. Removal of antibioties from wastewater by sewage treatment facilities in Hong Kong and Sheuzhen, China. Water research, 2008, vol. 42 (1–2), pp. 349–403.
7. Horzheyev V. M., Kotsyumbas I. Y., Kosenko Y. M., Tortkivska A. I., Zaruma L. E. Handbook of veterinary drugs. Lviv, publishing house “Afisha”, 2013, 1596 pp. (in Ukrainian)
8. Ivanova O. V., Zakharenko M. O. Sanitary sewage indicators pig enterprises for biological purification methods. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named S. Z. Gzhytsky, 2013, no. 3 (57), pp. 335–341. (in Ukrainian)
9. Kokunin V. A. Statistical data processing with a small number of trials. Ukrainian Biochemical Journal, 1975, issue 47, no. 6, pp. 776–790.
10. Kurbatova I. M The protein spectrum of blood plasma carp for the actions of chlortetracycline. Hydrobiological Journal, 2016, vol. 52, no. 2, pp. 103–108.
11. Kurbatova I. M, Tsedyk V. V. The quality of the water reservoirs of industrial fishing and its impact on the eggs of carp (Cyprinus carpio L.). Proceedings of Volyn Pedagogical University by L. Ukrainka, 2012, no. 9, pp. 224–228.
12. Laemmly U. K. Cleavage of structural proteins during the assembly of the heat of Bacteriophage T4.
Nature, 1970, vol. 227, no. 5259, pp. 680–685.
https://doi.org/10.1038/227680a013. Lukyanenko V. I. Environmental aspects of ichthyology. Moscow, “Agropromizdat”, 1987, 240 p. (in Russian)
14. Muradova G. R., Raadanova A. I. Dynamics of proteins in the blood serum of carp fingerlings in chronic effects of heavy metals. Successes of modern science, 2012, no. 7, pp. 58–62.
15. Reshetnikov D. S., Popov O. A., Koschulin N. A Evaluation of the well-being of the fishing community of water as a result of the morphological analysis of fish. The successes of modern biology, 1999, no. 2, pp. 165–177.
16. Shandrenko S. G., Golovin A. S., Dimitrenko M. P., Yurchenko A. I. The computer registration and analysis of TLC. Journal of chromatography of the Society, 2003, vol. 2, no. 4, pp. 22–30.
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