INFLUENCE OF APPLYING FEED SUPPLEMENTATION EXTRACTED FROM THE LEMNA MINOR ENRICHED WITH IODINE ON RATS’ BLOOD INDICATORS
S. V. Merzlov, R. O. Ryvak
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Bila Tserkva National Agrarian University,
8/1 Soborna sq., Bila Tserkva, Kyiv region, 09117, Ukraine
The article presents the results of the study of toxic effects of feed supplementation from freshwater algae Lemna minor enriched with iodine on the indicators of blood of white rats. The toxic effects of feeding were studied according to the OECD Guidelines for the testing of chemicals, Test no. 407 “Repeated Dose 28-Day Oral Toxicity Study in Rodents”. The additive was fed to two groups of animals with food daily during 28 days at a dose of 1000 mg/kg of animal body weight. The content of iodine in the additive group was 1537.4 mg/kg and 2574.5 mg/kg of dry biomass, respectively. Morphological studies of blood of laboratory animals were carried out At the beginning and At the end of the experiment. The content of hemoglobin, the total number of red blood cells and leukocytes, and differential count of leukocytes were monitored. Biochemical studies of blood serum were conducted on the following indicators: total protein content, amine nitrogen, creatinine, urea, enzyme activity (ALT, AST, ALP).
The total protein content, regardless of the feeding dose, was higher, although not significantly, compared to control. Increasing the content of amine nitrogen within the limits of the physiological norm during the experiment demonstrates the positive effect of the additive on the organism of laboratory animals and does not cause pathology of the gastrointestinal tract, fatty liver dystrophy, poisoning. During the experiment it was found that the feed additive does not have a negative effect on the morphological parameters of whole blood. From the data analysis of the biochemical parameters of blood of white rats, it was concluded that the feeding of the fodder additive from freshwater algae Lemna minor, enriched with iodine, does not have a toxic effect on the parameters of protein metabolism, activity of enzymes, indicating the absence of chronic disorders of the gastrointestinal tract, liver disease or toxicosis of various etiologies in the body.
Keywords: FEED ADDITIVE, LEMNA MINOR, DRY BIOMASS, IODINE, MORPHOLOGICAL PARAMETERS, BLOOD ENZYMES
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