Bìol. Tvarin, 2015, volume 17, issue 3, pp. 117–123
THE INFORMATIVENESS OF BIOCHEMICAL MARKERS CONTENT IN SERUM AT REPTILES METABOLIC OSTEOPATHY
G. O. Stepanenko
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Kharkiv State Zooveterinary Academy,
v. Mala Danilovka, Dergachi district, Kharkiv region, 62341, Ukraine
In this article the informativeness of biochemical markers of connective tissue status (glycoproteins, general chondroitinsulfates, fractions of glycosaminoglycans — chondroitin-6, -4- and keratansulfates) is submitted in different reptiles families at metabolic osteopathy. There were calculated the indexes for these tests of informativeness and grounded their values for assessment of the reptiles health at metabolic ostheopathy. On the base of this data it was found, that informativeness of calcium is 83.3 %, phosphorus — 100 %, alkaline phosphatase — 58.3 %, total chondroitinsulfates and glycosaminoglycans — 100 %, chondroitin-6-sulfate — 75% chondroitin-4-sulfate — 91.7 %, keratansulfates — 100 % in iguanas with metabolic osteopathy. Informativeness is 77.8 % for calcium, phosphorus — 55.6%, alkaline phosphatase and uric acid — 50 %, total chondroitinsulfates, glycosaminoglycans, chondroitin-6-sulfate and keratansulfates — 100 %, chondroitin-4-sulfate — 87.5 % at metabolic osteopathy in agamas. Increased uric acid level and AST activity in the serum of agamas with metabolic osteopathy testify about the development of endogenous intoxication in these reptile’s families and about more severe disease compared to iguanas. Thus, it was found that connective tissue diagnostic tests for metabolic osteopathy at iguans and agamas have been compared with traditional biochemical indicators in blood serum — calcium, phosphorus, total protein, activity of ALT, AST and alkaline phosphatase, urea, uric acid. We plan to create a diagnostic scheme for studying of reptiles metabolic osteopathy involving biochemical markers of connective tissue status for its assessment and subsequent correction.
Keywords: REPTILE, CONNECTIVE TISSUE, BIOCHEMISTRY, GLYCOPROTEINS, CHONDROITIN SULFATES, GLYCOSAMINOGLYCANS, INFORMATIVENESS
- Savinova I. V., Klestova Z. S. Viral infections reptiles — a real threat to the health of people and warm-blooded animals. Veterinary biotechnology: bulletin, 2012, no. 21, pp. 97–105. (in Ukrainian)
- Khairutdinov I. Z. Ecology reptiles urbanized areas (for example, Kazan): author. dis. on competition candidate. biol. sciences: spec. 03.02.08 “Ecology (biological sciences”. Kazan, 2010, 24 p. (in Russian)
- Jacobson E. R. Biology, husbandry, and medicine of the Green iguana. Krieger Publishing Co. Malabar, FL, 2009, 177 p.
- Hoby S., Wenker C., Robert N. Nutritional metabolic bone disease in juvenile veiled chameleons (Chamaeleo calyptratus) and its prevention. 2010, no. 140(11), pp. 1923–1931.
- Vasiliev D. B., Swed V. S. Prevention of disorders of mineral metabolism in reptiles in captivity and use vitamin and mineral supplements. Scientific researches in zoological parks. Moscow Zoo, 2009, no. 20, p. 15. (in Russian)
- Vasiliev D. B. Calcium and phosphorus metabolism in terrestrial vertebrates. Comparative pathology, differential diagnosis, therapy major, related and clinically similar diseases reptile. Materials X Intern. Vet. Congress, Moscow, 2012, pp. 134–152. (in Russian)
- Romer A. Osteology of the reptile. University of Chicago Press, Chicago, 2007, 800 p.
- Stadnik A. M., Fedorovich V. L. Modern trends in pre-clinical molecular diagnostics osteodystrophy. Scientific notes Vitebsk State. Acad. Vet. Medicine, 2007, vol. 43(1), pp. 228–230. (in Russian)
- Fedorovich V. L., Slivinska L. G. State of bone metabolism at osteodystrophy cows. Science. Journal of Lugansk National Agrarian University, Lugansk, 2011, no. 31, pp. 223–226. (in Ukrainian)
- Slivinska L. G., Fedorovich V. L. The content in blood markers of bone metabolism at osteodystrophy cows. Science. Bulletin Vet. Medicine, Bila Tserkva, 2011, vol. 8(87), pp. 151–155. (in Ukrainian)
- Vasiliev D. B. Osteorenal syndrome reptiles: features of the pathogenesis and therapy. Veterinary pathology, 2008, no. 2(17), pp. 85–89. (in Russian)
- Vasiliev D. B. Clinical and laboratory methods in the diagnosis of some internal diseases reptile. Mater. III International. Conf. “Actual problems of veterinary medicine of small animals in the North Caucasus”, 2000, Persianovskiy, pp. 28–31. (in Russian)
- Morozenko D.V., Levchenko V.I., Timoshenko O.P. Biochemical markers of connective tissue in the diagnosis of diseases of dogs and cats: guidance. Bila Tserkva, 2012, 42 p. (in Ukrainian)
