The Animal Biology

Bìol. Tvarin, 2015, volume 17, issue 3, pp. 43–51

PEROXIDATION PROCESSES IN THE BLOOD, LIVER AND SKELETAL MUSCLES OF RABBITS WITH ACUTE L-ARGININE-INDUCED PANCREATITIS AND ITS CORRECTION

O. O. Hopanenko, Y. F. Rivis

This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Agriculture of Carpathian Region National Academy of Agricultural Sciences
M. Hrushevskyi St., 5, Obroshyno, Lviv region., 81115, Ukraine

Aim of this study was to investigate the processes of peroxidation in the blood, liver and skeletal muscles of rabbits with acute L-Arginine-induced pancreatitis and its correction by feeding linseen oil.

The experiment was made in five groups of male rabbits (five animals in each group). The rabbits of the control and I, II, III and IV experimental groups received standard granulated feed during one month. However, during this period rabbits of the I and III experimental groups received daily feed with linseed oil, rabbits of the IV experimental group — feed with sunflower oil in the dose 1 ml/kg of body weight. Also 5 days before the end of the experiment rabbits of the control and experimental groups were made a single intraperitoneal injection of saline sodium chloride in dose 2 ml/kg of body weight and rabbits of the II, III and IV research groups were made an injection of L-arginine in a dose of 4 g/kg of body weight in the same amount of saline. As the material for the research samples of the blood, pancreas, liver and skeletal muscles were used.

It was found that after the intraperitoneal injection of L-arginine the number of necrotic acinar epithelial cells of the head and tail of the pancreas increases and the activity of lipase and α-amylase increases in the blood plasma of rabbits. Feeding up with linseed oil normalizes the number of necrotic acinar epithelial cells of the head and tail of the pancreas and activity of lipase and α-amylase in the blood plasma of rabbits; however feeding up with sunflower oil dramatically increases these indexes. For acute L-Arginine-induced pancreatitis superoxide dismutase and glutathione peroxidase greatly increases, but catalase decreases in the red blood cells, liver and skeletal muscles of the rabbits. The concentration of conjugated dienes, lipid hydroperoxides and malondialdehyde greatly increases in the blood plasma, liver and skeletal muscles of the rabbits with acute L-Arginine-induced pancreatitis. Feeding with linseed oil normalizes while feeding with sunflower oil enhances the vectors activity of superoxide dismutase, glutathione peroxidase and catalase in erythrocytes, liver and skeletal muscles and increases the amount of primary and secondary products of lipid peroxidation in the blood plasma, liver and skeletal muscles of the rabbits for acute L-Arginine-induced pancreatitis.

Keywords: BIOCHEMISTRY, RABBITS, PANCREATITIS, CORRECTION, BLOOD, LIVER, SKELETAL MUSCLE, PANCREAS STATE, PEROXIDATION PROCESSES

1. Chernobrovyj V. M., Fedzhaga I. V. The role of gastric secretion in pathogenesis of chronic pancreatitis. Bukovinsky Medical Journal, 2008, 12, no. 1, pp. 156–162. (in Ukrainian)
2. Shmanko V. V., Mereczka I. V. Clinical and pharmacological aspects of enzymes in gastroenterology. Medicaments of Ukraine, 2008, 119, no. 3, pp. 82–84. (in Ukrainian)
3. Kopelnyuk V., Galenova T., Kot L., Bogdanova O., Ostapchenko L. The role of insulin in the regulation of carbohydrate and lipid metabolism by conditions of metabolic syndrome. Journal of Kyiv National University of Taras Shevchenko, 2010, 56, pp. 15–16. (in Ukrainian)
4. Iskra R. Ya. Insulin and lipid content in blood plasma of pigs at increase of chromium level in their diet. The Animal Biology, 2009, vol. 11 (1–2), pp. 176–179. (in Ukrainian)
5. Eydoux C., Aloulou A., De Caro J., Grandval P., Laugier R., Carrière F., De Caro A. Human pancreatic lipase-related protein 2: Tissular localization along the digestive tract and quantification in pancreatic juice using a specific ELISA. Biochimica et Biophysica Acta (BBA), 2006, 1760, no. 10, pp. 1497–1504.
6. Homan R., Jain M. K. Biology, pathology, and interfacial enzymology of pancreatic phospholipase A₂. Intestinal Lipid Metabolism, 2001, pp. 81–104.
7. Namkung W., Han W., Luo X., Muallem S., Cho K. H., Kim K. H., Lee M. G. Protease-activated receptor 2 exerts local protection and mediates some systemic complications in acute pancreatitis. Gastroenterology, 2004, 126, no. 7, pp. 1844–1859.
8. Motta J.-P., Martin L., Vergnolle N. Proteases/antiproteases in inflammatory bowel diseases. Proteases and Their Receptors in Inflammation, 2011, pp. 173–215.
9. Singh V. K., Wu B. U., Bollen T. L., Repas K., Maurer R., Mortele K. J., Banks P. A. Early systemic inflammatory response syndrome is associated with severe acute pancreatitis. Clinical Gastroenterology and Hepatology, 2009, 7, no. 11, pp. 1247–1251.
10. Mayerle J., Simon P., Lerch M. M. Medical treatment of acute pancreatitis. Gastroenterology Clinics of North America, 2004, 33, pp. 855–869.
11. Windsor A. C., Kanwara S., Li A. G., Barnes E., Guthrie J. A., Spark J. I., Welsh F., Guillou P. J., Reynolds J. V. Compared with parenteral nutrition, enteral feeding attenuates the acute phase response and improves disease severity in acute pancreatitis. Gut, 1998, 42, pp. 431–435.
12. Büchler M. W., Gloor B., Müller C. A., Friess H., Seiler C. A., Uhl W. Acute necrotizing pancreatitis: treatment strategy according to the status of infection. Annals of Surgery, 2000, 232, no. 5, pp. 619–626.
13. Balani A. R., Grendell J. H. Drug-induced pancreatitis. Incidence, management and prevention. Drug Safety, 2008, 31, no. 10, pp. 823–837.
14. Eland I. A., Alvarez C. H., Stricker B. H., Rodriguez L. A. The risk of acute pancreatitis associated with acid-suppressing drugs. British Journal of Clinical Pharmacology, 2000, 49, no. 5, pp. 473–478.
15. Economou M., Zissis M. Infectious cases of acute pancreatitis. Annals of Gastroenterology, 2000, 13, no. 2, pp. 98–101.
16. Zhang X., Qi R., Xian X., Wang Y., Huang W., Liu G. Atherogenesis, pancreatitis and brain dysfunction in LPL deficient mice with severe hypertriglyceridemia. Atherosclerosis Supplements, 2008, 9, no. 1, p. 29.
17. Naito Z., Ishiwata T., Lu Y. P., Teduka K., Fujii T., Kawahara K., Sugisaki Y. Transient and ectopic expression of lumican by acinar cells in L-arginine-induced acute pancreatitis. Experimental and Molecular Pathology, 2003, 74, no. 1, pp. 33–39.
18. Pryvroczka I. B., Pokotylo O. S. Dynamics of pro- and antioxidant balance in acute pancreatitis and its correction. Experimental and Clinical Physiology and Biochemistry, 2011, no. 2, pp. 42–47. (in Ukrainian)
19. Tverdokhlib I. V., Stepanov Iu. M., Sirenko O. Iu., Zinenko D. Iu., Berehovenko I. M. Structural and functional changes of liver’s microcirculation in a rat model of acute pancreatitis induced by sodium taurocholate. Morphology, 2011, 5, no 3, pp. 71–74. (in Ukrainian)
20. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of research in biology, veterinary medicine: a guide. Lviv, Spolom Publ., 2012, 764 p. (in Ukrainian)
21. Lapach S. N., Chubenko A. V., Babych P. N. Statistical methods in biomedical research using Excel. Kyiv, Morion Publ., 2001. 408 p. (in Russian)
22. Rochette L., Lorin J., Zeller M., Guilland J-C., Lorgis L., Cottin Y., Vergely C. Nitric oxide synthase inhibition and oxidative stress in cardiovascular diseases: Possible therapeutic targets? Pharmacology & Therapeutics, 2013, 140, no. 3, pp. 239–257.
23. Sirenko O. Iu., Tverdokhlib I. V., Stepanov Iu. M. Morphological study of liver and pancreas parenchyma in conditions of L-arginine-induced acute pancreatitis. Morphology, 2011, 5, no. 2, pp. 67–74. (in Ukrainian)
24. Szabó S., Ischiropoulos H., Radi R. Peroxynitrite: biochemistry, pathophysiology and development of therapeutics. Nature Reviews, 2007, 6, pp. 662–680.
25. Bozza P. T., Bakker-Abreu I., Navarro-Xavier R. A., Bandeira-Melo C. Lipid body function in eicosanoid synthesis: An update. Prostaglandins Leukotrienes Essential Fatty Acids, 2011, 85, pp. 205–213.
26. Toma H., Winston J., Micci M-A., Shenoy M., Pasricha P. J. Nerve growth factor expression is up-regulated in the rat model of L-arginine-induced acute pancreatitis. Gastroenterology, 2000, 119, no 5, pp. 1373–1381.
27. Treacy J, Williams A, Bais R, Willson K, Worthley C, Reece J, Bessell J, Thomas D. Evaluation of amylase and lipase in the diagnosis of acute pancreatitis. ANZ J. Surg., 2001, 71, no. 10, pp. 577–582.
28. Dawra R., Sharif R., Phillips P., Dudeja V., Dhaulakhandi D., Saluja A. K. Development of a new mouse model of acute pancreatitis induced by administration of L-arginine. American Journal of Physiology — Gastrointestinal and Liver Physiology, 2007, 292, no. 4, pp. G1009–G1018.
29. Varga I. S., Matkovics B., Czako L., Hai D. Q., Kotorman M., Takacs T., Sasvari M. Oxidative stress changes in L-Arginine-induced pancreatitis in rats. Pancreas, 1997, 14, no. 4, pp. 355–359.

Download full text in PDF