Laboratory of molecular biology and clinical biochemistry, Institute of Animal Biology NAAS: the history and present state

V. V. Vlizlo, D. D. Ostapiv, I. M. Petrukh, M. R. Kozak, N. V. Kuzmina, Yu. V. Martyn

prion_This email address is being protected from spambots. You need JavaScript enabled to view it., This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Animal Biology NAAS,
38 V. Stus, str., Lviv, 79034, Ukraine

The Laboratory of molecular biology and clinical biochemistry was established with the foundation of Scientific Production Center (SPC) for the study of prion infections in 2001. SPC for the study of prion infections was founded by orders of the Ukrainian Academy of Agrarian Sciences and the State Department of Veterinary Medicine of Ukraine. Scientists of the laboratory provide scientific support of the livestock products production based on studies of animal metabolism peculiarities according to the norms and internal pathology, study of the mechanisms of prion infection development, biological aspects and application of cell culture techniques in new veterinary drug discovery, development of nanocarriers with adjuvant properties, physiological and biochemical processes in semen. The conducted researches allow an improvement of the production profitability, livestock safety, reproductive capacity and live weight gain. For the reduction of the cost of livestock production the laboratory offers advanced diagnostic tests, effective measures for the treatment and prevention of metabolic diseases of high yielding cows, new generation tools for the development of vaccines and drugs, methods of treatment and prevention of BSE, methods for assessing and normalizing metabolic processes in ejaculate to increase the fertility of sperm.

Key words: laboratory of molecular biology and clinical biochemistry, Institute of Animal Biology NAAS, history, present, employees, work directions, results, prospects

1. Antoniak HL, Vlizlo VV. Biochemical and geochemical role of iodine. A monograph. Lviv, Ivan Franko National University of Lviv, 2013: 392 p. (in Ukrainian)
2. Branets RM, Kuzmina NV, Denis HG, Martin YV. Physiological and biochemical parameters of blood when feeding rats metal complexes in the composition of the polymer transporter and nutrient-deficient diet. Scientific and technical bulletin of State scientific research control institute of veterinary medical products and fodder additives and Institute of Animal Biology. 2019; 20 (2): 34–42. (in Ukrainian)
3. Chekh BO, Ferens MV, Ostapiv DD, Samaryk VY, Varvarenko SM, Vlizlo VV. Characteristics of novel polymer based on pseudo-polyamino acids GluLa-DP G-PE G600: binding of albumin, biocompatibility, biodistribution and potential crossing the blood-brain barrier in rats. Biochem. J. 2017; 89 (4): 13–21. https://doi.org/10.15407/ubj89.04.013
4. Gerilovich AP, Stegniy BT, Zavgorodniy AI, Vlizlo VV. Molecular genetic diagnostic methods in veterinary medicine and biotechnology. Ed. by Gerilovich A.P. and Stegniy B.T. Kyiv, ST-Print. 2014: 286 p. (in Ukrainian)
5. Ibatullin II, Zhukorsky OM, Vlizlo VV. Methodology and organization of scientific research in animal husbandry. Kyiv, Ahrarna nauka. 2017: 328 p. (in Ukrainian)
6. Iskra RY, Vlizlo VV, Fedoruk RS, Antoniak HL. Chromium in the nutrition of animals. Kyiv, Ahrarna nauka, 2014: 312 p. (in Ukrainian)
7. Kozak MR, Oliynyk AV, Zaichenko OS, Vlizlo VV. Adjuvant properties of polymer based on acrylic acid. Biochem. J. 2013; 85 (3): 69–73. (in Ukrainian) https://doi.org/10.15407/ubj85.03.069
8. Kozak MR, Vlizlo VV. Comparison of immunological action of classical, synthetic and natural adjuvants. Chem. 2019; 21 (3): 194. (in Ukrainian)
9. Kushkevych M, Vlizlo V. Relationship between the cellular prion level and ATPases activities in the liver and kidneys of different age Wistar line rats. Bìol. Tvarin. 2019; 21 (2): 112.
10. Kushkevych MV, Vlizlo VV. Localization and level of the cellular prion in the jejunum of the rats Wistar line of different age groups. Bilogical systems. 2013; 3: 325–329.
11. Kuzmina NV, Ostapiv DD, Chajkovska A, Panych A. The activity and isoforms of superoxide dismutase in tisues of reproductive organs of rats. Scientific and technical bulletin of State scientific research control institute of veterinary medical products and fodder additives and institute of animal biology. 2020; 21 (1): 113–118. (in Ukrainian) https://doi.org/10.36359/scivp.2020-21-1.13
12. Levchenko VI, Vlizlo VV, Kondrakhin IP. Veterinary clinical biochemistry. Ed. by VI Levchenko and VL Galas. Bila Tserkva, 2002, 2019. (in Ukrainian)
13. Levchenko VI, Vlizlo VV, Kondrakhin IP. Clinical diagnosis of animal internal diseases. Ed. by VI Levchenko. Bila Tserkva, 1995, 2004, 2017 (in Ukrainian)
14. Levchenko VI, Vlizlo VV, Kondrakhin IP. Internal animal diseases. Ed. by VI Levchenko. Bila Tserkva, 1999, 2001, 2012, 2015. (in Ukrainian)
15. Schkilnyk O, Petrukh I, Ostapiv D, Kozak M, Vlizlo V, Pavlyuk I, Vezdenko O, Sekh O, Ponomarjova S, Kalynovska L. Effect of feed additives on mineral homeostasis of cows with ketosis. Scientific and Technical Bulletin of State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives and Institute of Animal Biology. 2020; 21 (1): 247–251. (in Ukrainian) https://doi.org/10.36359/scivp.2020-21-1.31
16. Simonov M, Vlizlo V. Some blood markers of the functional state of liver in dairy cows with clinical ketosis. J. Vet. Med. 2015; 18 (1): 74–82. https://doi.org/10.15547/bjvm.814
17. Simonov M, Vlizlo V. The effect of “Remivital” on plasma amino acid composition in dairy cows with ketosis. Sci. Pract. 2016; 3 (1): 73–79. https://doi.org/10.15407/agrisp3.01.073
18. Stadnyk VV, Izumova LA, Rzhepetskiy YA, Mayor CY, Vlizlo VV. The Antisense Oligonucleotides Decrease Expression of the Cellular Prion. Letters in Drug Design & Discovery. 2010; 7 (1): 23–26. https://doi.org/10.2174/157018010789869299
19. Stadnyk V, Mayor C, Izyumova L, Vlizlo V. Relation between duration of incubation period of prion infections and prion protein conformation. Medical Hypotheses. 2011; 77 (2): 199–200. https://doi.org/10.1016/j.mehy.2011.04.010
20. Stegniy BT, Gerilovich AP, Ibitulin II, Bisyuk IY, Komisarenko SV, Vlizlo VV. Problems of biological safety and biological protection in veterinary medicine and biotechnology. Kharkiv, HTMT, 2013: 414 p. (in Ukrainian)
21. Stetsyshyn Y, Raczkowska J, Harhay K, Awsiuk K, Shymborska Y, Nastyshyn S, Ohar H, Vasilyev V, Ostapiv D, Sharan M, Sharan O, Voronov S, Budkowski A. Grafted polymer brush coatings for growth of cow granulosa cells and oocyte-cumulus cell complexes. Biointerphases. 2020; 15 (3): 031006. https://doi.org/10.1116/6.0000183
22. Strus O, Polovko N, Ostapiv D, Kuzmina N. Study of the toxicity of drugs with sapropel extracts and the testing of the cream effectiveness. Norwegian Journal of Development of the International Science. 2019; 1 (32): 37–42.
23. Verbytsky PI, Gorzheev VM, Kucheryavenko OO, Vlizlo VV. Instructions for the diagnosis, prevention and control of bovine spongiform encephalopathy. Kyiv, 2002: 7 p. (in Ukrainian)
24. Vlizlo V, Fedoruk R. Science of the western region of Ukraine (1990–2010). Lviv, PAIS, 2011: 354–361. (in Ukrainian)
25. Vlizlo V, Iskra R, Maksymovych I, Berezovskyy R. The system of erythrocyte antioxidant protection in piggery as affected by ferrous citrate. British Journal of Science, Education and Culture. 2014; 1 (5): 44–49.
26. Vlizlo V, Iskra R, Maksymovych I, Lis M, Niedziółka Disturbance of antioxidant protection and natural resistance factors in rats with different availability of trivalent chromium. Turk. J. Vet. Anim. Sci. 2014; 38: 138–144. https://doi.org/10.3906/vet-1305-44
27. Vlizlo VV. Instructions for the prevention and control of bluetongue. Kyiv, 2009: 6 p. (in Ukrainian)
28. Vlizlo VV, Fedoruk RS, Ratich IB. Laboratory research methods in animal biology and veterinary medicine. Ed. by VV Vlizlo. Lviv, Spolom, 2012: 764 p. (in Ukrainian)
29. Vlizlo VV, Kurtiak BM, Vudmaska IV, Vishchur OI, Petruk AP. Fat-soluble vitamins in veterinary medicine and animal husbandry. A monograph. Lviv, 2015: 436 p. (in Ukrainian)
30. Vlizlo VV, Maksimovich IA, Galyas VL, Lenyo MI. Laboratory diagnostics in veterinary medicine. Lviv, 2008: 2014. (in Ukrainian)
31. Vlizlo VV. Nanobiotechnologies and nanoproducts: achievements and prospects of research in animal husbandry and veterinary medicine. Agricult. Sci. 2017; 5: 5–10.
32. Vlizlo V, Petruh I, Simonov M, Slivinska L. Hormonal regulation of energy metabolism in ketotic cows. XVIII Middle-European Buiatrics Congress, Eger, Hungary, 2018: 294−298.
33. Vlizlo V, Verbytsky P, Kulyk M. National standard of Ukraine: Prion infections. Methods of sampling and preparation of brain samples for laboratory diagnosis. DSTU 7070:2009. Kyiv. 2010: 10 p. (in Ukrainian)
34. Zelenina OM, Ostapiv DD, Vlizlo VV, Dron IA, Vinnytska Hematological indices of rats after administration of enrofloxacin as a subunit of polymer. Bìol. Tvarin. 2020; 22 (1): 26–30. (in Ukrainian) https://doi.org/10.15407/animbiol22.01.026