Bìol. Tvarin. 2021; 23 (2): 37–40.
Received 23.05.2021 ▪ Accepted 25.06.2021 ▪ Published online 01.07.2021

Effect of hop cones and vitamin E on ketogenesis and antioxidant status in transition dairy cows

S. R. Sachko1, I. V. Vudmaska1, I. V. Nevostruyeva1, R. G. Sachko1, A. P. Petruk2

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1Institute of Animal Biology NAAS,
38 V. Stus str., Lviv, 79034, Ukraine

2Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv,
50 Pekarska str., Lviv, 79010, Ukraine

The purpose of the work was the correction rumen fermentation in the transition cows to prevent metabolic disorders. For the experiment, two groups of Ukrainian dairy black-and-white breed cows were formed, 10 animals per group. The experiment lasted 3 weeks prepartum and 3 weeks postpartum. Animals received a balanced diet, which consisted of haylage, silage, barley, wheat, corn, soybean meal, salt, mineral and vitamin premix. The first group was the control. To the diet of second group 300 mg of α-tocopherol acetate (0.6 g of Rovimix E-50) and 1 g/kg of dry hop cones per kg of dry matter was added. Before calving, the tested feed additive reduced the concentration of peroxide oxidation products in the cows blood (P<0.05) without affecting other parameters. Changes that are more significant detected after calving. A decrease in the concentration of lipid hydroperoxides (P<0.05), TBARS (P<0.05), and beta-hydroxybutyrate (P<0.05) were observed in the blood of the cows of the experimental group. Therefore, the addition into diet of transition cows of α-tocopherol and hop cones inhibits the lipid peroxidation and reduces the ketones formation. So, this feed supplement can be used to prevent ketosis and steatosis in cows.

Key words: cows, hop cones, vitamin E, blood, ketone bodies, peroxidation

  1. Behr J, Vogel RF. Mechanisms of hop inhibition include the transmembrane redox reaction. Appl. Environ. Microbiol. 2010; 76 (1): 142–149. DOI: 10.1128/AEM.01693-09.
  2. Compton CW, Young L, McDougall S. Efficacy of controlled-release capsules containing monensin for the prevention of subclinical ketosis in pasture-fed dairy cows. New Zeal. Vet. J. 2015; 63 (5): 249–253. DOI: 10.1080/00480169.2014.999842.
  3. Flythe MD. The antimicrobial effects of hops (Humulus lupulus L.) on ruminal hyper ammonia-producing bacteria. Lett. Appl. Microbiol. 2009; 48 (6): 712–717. DOI: 10.1111/j.1472-765X.2009.02600.x.
  4. Hartkorn A, Hoffmann F, Ajamieh H, Vogel S, Heilmann J, Gerbes AL, Vollmar AM, Zahler S. Antioxidant effects of xanthohumol and functional impact on hepatic ischemia-reperfusion injury. J. Nat. Prod. 2009; 72 (10): 1741–1747. DOI: 10.1021/np900230p.
  5. Hop acids as a replacement for antibiotics in animal feed. Patent no. US8197863B2. Date of Patent: 12.06.2012. Available at: https://patents.google.com/patent/US8197863B2/en
  6. Karabín M, Hudcová T, Jelínek L, Dostálek P. Biologically active compounds from hops and prospects for their use. Comprehen. Rev. Food Sci. Food Saf. 2016; 15 (3): 542–567. DOI: 10.1111/1541-4337.12201.
  7. Krofta K, Mikyška A, Hašková D. Antioxidant characteristics of hops and hop products. J. Inst. Brew. 2008; 114 (2): 160–166. https://doi.org/10.1002/j.2050-0416.2008.tb00321.x
  8. Markantonatos X, Varga GA. Effects of monensin on glucose metabolism in transition dairy cows. J. Dairy Sci. 2017; 100 (11): 9020–9035. DOI: 10.3168/jds.2016-12007.
  9. McGuffey RK. A 100-year review: Metabolic modifiers in dairy cattle nutrition. J. Dairy Sci. 2017; 100 (12): 10113–10142. DOI: 10.3168/jds.2017-12987.
  10. Narvaez N, Wang Y, Xu Z, McAllister T. Effects of hops on in vitro ruminal fermentation of diets varying in forage content. Livestock Sci. 2011; 138 (1–3): 193–201. DOI: 10.1016/j.livsci.2010.12.028.
  11. Olas B, Kolodziejczyk J, Wachowicz B, Jędrejek D, Stochmal A, Oleszek W. The extract from hop cones (Humulus lupulus) as a modulator of oxidative stress in blood platelets. Platelets. 2011; 22 (5): 345–352. DOI: 10.3109/09537104.2010.549597.
  12. Politis I. Reevaluation of vitamin E supplementation of dairy cows: bioavailability, animal health and milk quality. Animal. 2012; 6 (9): 1427–1434. DOI: 10.1017/S1751731112000225.
  13. Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory methods of research in biology, animal husbandry and veterinary medicine. A reference book. Lviv, Spolom. 2012; 764 p. (in Ukrainian)
  14. Vudmaska I, Petruk A, Vaskiv R, Vlizlo V. Comparison of monensin and hop cones effects on rumen fermentation and blood parameters in transition dairy cows. XVIII Middle-European Buiatrics Congress. Hung. Vet. J. 2018; 140 (S1): 299–304.
  15. Wankhade PR, Manimaran A, Kumaresan A, Jeyakumar S, Ramesha KP, Sejian V, Rajendran D, Varghese MR. Metabolic and immunological changes in transition dairy cows: A review. Vet. World. 2017; 10 (11): 1367–1377. DOI: 10.14202/vetworld.2017.1367-1377.
  16. Weiskirchen R, Mahli A, Weiskirchen S, Hellerbrand C. The hop constituent xanthohumol exhibits hepatoprotective effects and inhibits the activation of hepatic stellate cells at different levels. Front. Physiol. 2015; 6: 140. DOI: 10.3389/fphys.2015.00140.

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