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Bìol. Tvarin. 2022; 24 (4): 12–16.
https://doi.org/10.15407/animbiol24.04.012
Received 11.06.2022 ▪ Revision 05.10.2022 ▪ Accepted 16.11.2022 ▪ Published online 30.12.2022

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Hematological indicators and sperm quality of rams during the sexual rest period when fed a liposomal vitamin and mineral supplement

O. M. Sharan, V. Yu. Stefanyk

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Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv,
50 Pekarska str., Lviv, 79010, Ukraine

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The aim of the work was to investigate the effect of feeding a liposomal vitamin and mineral supplement during the period of sexual rest on hematological indicators and quality of ram sperm. The experiment was conducted at the PE “Kogut BM” in Horodotsky district, Lviv region, on 12 clinically healthy Texel rams, aged 2–4 years, during the period of sexual rest (March-May). Animals were divided into two groups — control and experimental, 6 animals per group. The control rams received the basic diet containing hay, corn silage, and compound feed. For 45 days, the sheep of the experimental group were individually added to the combined feed a feed additive containing vitamins A, D₃, E, C and zinc gluconate in the form of a liposomal emulsion. At the beginning and at the end of feeding, we took blood samples, in which hematological indicators were determined. After the end of supplement feeding, ejaculates were collected twice a week in doublets for three weeks. Physiological indicators of ejaculate quality (volume, sperm concentration, percentage of live sperm, as well as sperm viability, morphological disorders and percentage of degenerative sperm) were determined using the CASA computerized system. It was established that feeding liposomal vitamin-mineral supplements to rams during the period of sexual rest improves hematological indicators: the content of erythrocytes, hemoglobin, platelets and hematocrit increase significantly (P<0.05–0.001) while the content of leukocytes decreases by 24.2% (P<0.05). Accordingly, the erythrocyte indices of the blood of experimental breeding rams were higher than those of control animals. Feeding rams with a liposomal vitamin and mineral supplement ensured an increase in ejaculate volume by 17.6% (P<0.05), sperm concentration (P<0.01), their viability, as well as a decrease in the number of immature (P<0.001) and degenerated (P<0.05) sperm. Higher quality indicators of ejaculates of rams under the influence of vitamins A, D₃, E, C and zinc gluconate indicate the possibility of obtaining sperm from breeding rams during the period of sexual rest.

Key words: vitamin and mineral supplement, liposomal emulsion, ram, hematological indicators, sperm, survival, fertilizing capacity, period of sexual rest

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