FATTY ACID COMPOSITION IN SKELETAL MUSCLE OF FATTENING BULLS FED DIETS WITH DIFFERENT LEVEL OF VITAMIN E AND SELENIUM
Y. P. Bilash1, 2, A. P. Didovych2, I. V. Vudmaska1, 2, O. V. Holubets3
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1Institute of Animal Biology NAAS; 38 V. Stus St, Lviv, 79034, Ukraine
2Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky; 50 Pekarska St, Lviv, 79010, Ukraine
3All-Ukrainian state research and production center for standardization, metrology, certification and consumers’ rights protection; 4 Metrologichna St, Kyiv, 03143, Ukraine
Selenium and vitamin E have not only antioxidant effects in animal organism but also affect the activity of microorganisms includingin rumen. Change the value of species and enzyme activity of rumen microorganisms impacts on synthesis of specific bacterial fatty acids and on dietary polyunsaturated fatty acids hydrogenation.
Experiment on the 3-groups of Ukrainian black and white dairy breed bulls with 5 animals in each group has been provided. The control group fed a balanced diet. Bull of the 1st experimental group received the same diet with the addition of 0.3 mg/kg of selenium (sodium selenite) and 100 mg of vitamin E, and 2nd experimental group — 0.5 mg/kg of selenium (sodium selenite) and 300 mg of vitamin E per 1 kg DM. The experiment lasted 2 months with subsequent slaughter at 18 months age. Fatty acids profile was studied by gas chromatograph HP-6890 with 100 m length capillary column SP-2560.
In the lipids of musculus longissimus dorsi of the control, 1st and 2nd research groups were found (%): branched-chain acids — 0.82, 0.87, 1.12 (p<0.001); odd-chain acids — 3.67, 3.86, 4.29 (p<0.01); linoleic acid — 3.79, 4.21
(p<0.05), 6.47 (p<0.001); linolenic acid — 1.47, 1.45, 2.44 (p<0.001); trans10-18:1 — 1.09, 0.92, 0.77 (p<0.05); trans11-18:1 — 2.09, 2.39, 3.05 (p<0.05); trans10,cis12-18:2 — 0.11, 0.06 (p<0.05), 0.05 (p<0.001), cis9,trans11-18:2 — 0.30, 0.32, 0.47 (p<0.01). Quantities of acids 20:4, 20:5, 22:5 and 22:6 were increased in the muscle of 2nd experimental group bulls. The contents of palmitic, stearic and oleic acids did not change.
Thus, supplementation of bulls diet with high doses of selenium and vitamin E (2nd experimental group) changed the levels of formed in the rumen fatty acids, including conjugated linoleic acid. In addition, number of polyunsaturated fatty acids was increased, what may be associated with a lesser degree of peroxidation in the tissues. A lower dose of selenium and vitamin E (1st experimental group) affected on the fatty acid composition of muscle tissue much less.
Key words: BULLS, SELENIUM, VITAMIN E, SKELETAL MUSCLE, LONG-CHAIN FATTY ACIDS
