RELATIONS BETWEEN ACTIVITY AND CONTENT OF ENZYME MALATE-ASPARTATE shuttle ISOFORMS IN THE EJACULATE AND SURVIVAL OF SPERMATOZOA
N.V. Kuzmina
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Institute of animal biology of NAAS, Lviv, 38 Stus Str. 79034,Ukrainian
We studied the correlation between the activity and malate dehydrogenase (MDH) and aspartate aminotransferase (AST) isoform content and the survival of human, boar and bull sperm. It is found that the activity of MDH and AST in the ejaculate exhibits species specificity and are, respectively: human, 0,35±0,09 and 70,0±5,10; boar — 0,27±0,09 and 53,3±8,8 and bull 28,0±5,03 and 63,2±6,30 nmol/min× mg protein. Content of MDH1 isoform shows a weak species specificity (21,5−28,2 %), MDH2 — significantly higher (77,2±5,77 % — a man and 22,2–28,2 % in boar and bull) MDH3 is absent in man and in boar and bull almost identical (50,3 and 49,8 %). Content of AST1 in male ejaculate is high (59,2±10,10 %) and lower in boar and bull by 7,5–7,6 % (p > 0,05) and, conversely, AST2 in boar and bull sperm — higher (48,3–48,4 %) and lower for 7,5–7,6 % (p > 0,05) in human. There is a strong positive relationship between MDH activity and the survival of spermatozoa in ejaculates of boar (η = 0,807) and bull (η = 0,745) and average relationship force in man (η = 0,559). Between the survival of spermatozoa and AST activity there is a strong positive correlation in men ejaculates (η = 0,742), moderate strength — in bull (η = 0,330) and a negative average force — in boar (η = 0,532). Correlation relation between the content of AST1 isoforms and survival of sperm in all males is positive: in human — η = 0,657, bull — η = 0,639 and boar — η = 0,769 and content of AST2 — negative, respectively: η = 0,740; 0,586; 0,722. Content of MDH1 isoform shows a strong positive correlation with spermatozoa survival in human (η = 0,843), moderate strength — boar (η = 0,688) and weak —- bull (η = 0,254). There is a negative correlation between content of MDH2 isoforms and survival of sperm: human, boar and bull, respectively, η = 0,559; 0,846 and 0,555. MDH3 shows a strong positive correlation with the survival of sex cells in the boar (η = 0,785) and average force in bull (η = 0,639) ejaculates. Strong positive correlation between the activity of enzymes and their isoforms and spermatozoa survival indicates the need for the use of studied biochemical parameters to evaluate the physiological quality and insemination forecasting ability of germ cells.
Key words: ASPARTATE DEHYDROGENASE, MALATE DEHYDROGENASE, ISOFORM, SPERMATOZOA SURVIVAL, CORRELATION RATIO
