Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria

O. N. Chechet¹, V. L. Kovalenko², T. O. Garkavenko¹, O. I. Gorbatyuk¹, T. H. Kozytska¹

This email address is being protected from spambots. You need JavaScript enabled to view it.

¹State Research Institute for Laboratory Diagnostics and Veterinary Sanitary Examination,
30 Donetska str., Kyiv, 03151, Ukraine

²State Scientific Control Institute of Biotechnology and strains microorganisms,
30 Donetska str., Kyiv, 03151, Ukraine

The article presents the results of the new developed disinfectant “Biolide” research for use in poultry and other sectors of agriculture, because today the problem of developing cheap and effective disinfectants remains relevant. In recent years, the poultry industry has a tendency to rapid development, since the population’s demands for poultry products have increased markedly. With an integrated approach to the production of chicken meat, it is possible to increase the productivity of poultry enterprises, energy efficiency and reduce the cost of production. When applying such an integrated approach, one of the important roles is played by the provision of high-quality disinfection with effective and inexpensive means. In connection with the relevance of the development of new effective disinfectants, the main purpose of the research was to determine the effectiveness of working solutions in concentrations of 0.1; 0.2; 0.25 and 0.5% for gram-negative E. coli ATCC 25922 and gram-positive S. aureus ATCC 25923 for different periods of time — 20, 30, 60 and 120 min. after the simulation of protein contamination. Test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 in lyophilized form were stored in a refrigerator at a temperature –70±5°C. By replacing them on nutrient media, metabolic processes were restored and their correspondence to the main typical properties for this type of pathogens was checked. Simulation of protein contamination was carried out using sterile inactivated blood serum of cattle in the amount of 40.0% to the volume of the used bacterial suspension. In laboratory tests, smooth surfaces of tiles with an area 100 cm² were used as test objects. The analysis of the obtained research results showed the high efficiency of 0.5% working solutions of the new disinfectant “Biolide” when exposed to test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 for 60 min., since this concentration of the working disinfectant solution and the exposure time ensured the destruction by 99.99–100.0% of gram-negative and gram-positive microorganisms when imitating protein contamination of test objects.

Key words: “Biolide” disinfectant, working solutions, protein contamination, E. coli ATCC 25922 test culture, S. aureus ATCC 25923 test culture, test objects

1. Addie DD, Boucraut-Baralon C, Egberink H, Frymus T, Gruffydd-Jones T, Hartmann K, Horzinek MC, Hosie MJ, Lloret A, Lutz H, Marsilio F, Pennisi MG, Radford AD, Thiry E, Truyen U, Möstl K. Disinfectant choices in veterinary practices, shelters and households: ABCD guidelines on safe and effective disinfection for feline environments. Feline Med. Surg. 2015; 17 (7), 594–605. DOI: 10.1177/1098612X15588450.
2. Fotina GA. Determination of the toxicity of the drug “Bi-des” for the disinfection of poultry objects. messenger LNUVMBT S. Z. Gzhytsky. Vet. ser. 2014; 16; 2 (1): 340–347. (in Ukrainian)
3. Harkavenko TO, Kovalenko VL, Horbatiuk OI, Pinchuk NH, Kozytska TH, Harkavenko VM, Ordynska DO. Methodical recommendations for the purpose of bactericidal activity and control of the presence of bacteriostatically effective disinfectants. Kyiv, DNDILDZBEVSE. 2020. (in Ukrainian)
4. Hoshko KO. Analisis of efficiency of the methods disinfection and sterilization in dental. Medicine and Pharmacy. Scientific Collection “InterConf+”: International scientific discussion: problems, tasks and prospects, June 21–22. 2021; 6: 238–245. DOI: 10.51582/interco21-22.06.2021.26. (in Ukrainian)
5. Ivchenko VM (ed.). The handbook on sanitary-microbiological methods for research of food products and environmental objects. Bila Tserkva, 2004: 242 p. (in Ukrainian)
6. Kotsiumbas IY, Tishyn OL, Khomiak RV. Bacteriological properties of Aerosan Sci. Tech. Bulletin SSRCI Vet. Med. Prod. Feed Add. 2012; 13 (3–4): 211–214. (in Ukrainian)
7. Kovalenko VL, Ponomarenko GV, Kukhtyn MD, Midyk SV, Horiuk YV, Garkavenko VM. Changes in lipid composition of Escherichia coli and Staphylococcus aureus cells under the influence of disinfectans Barez, Biochlor and Geocide. J. Ecol. 2018; 8 (1): 547–550. DOI: 10.15421/2018_248. (in Ukrainian)
8. Kovalenko VL, Ponomarenko GV, Kukhtyn MD, Paliy AP, Bodnar OO, Rebenko HI, Kozytska TG, Makarevich TV, Ponomarenko OV, Palii AP. Evaluation of acute toxicity of the “Orgasept” disinfectant. J. Ecol. 2020; 10 (4): 273–278. DOI: 10.15421/2020_199. (in Ukrainian)
9. Kuo J. Disinfection processes. Water Environ. Res. 2017; 89 (10): 1206–1244. DOI: 10.2175/106143017X15023776270278.
10. Kwon D, Lim YM, Kwon JT, Shim I, Kim E., Lee DH, Yoon B, Kim P, Kim HM. Evaluation of pulmonary toxicity of benzalkonium chloride and triethylene glycol mixtures using in vitro and in vivo Environ. Toxicol. 2019; 34 (5): 561–572. DOI: 10.1002/tox.22722.
11. Li X, Wu Z, Dang C, Zhang M, Zhao B, Cheng Z, Chen L, Zhong Z, Ye Y, Xia Y. A metagenomic-based method to study hospital air dust resistome. Eng. J. 2021; 406: 126854. DOI: 10.1016/j.cej.2020.126854
12. Liulin PV, Severin RV, Gontar AM. Research of sorption and disinfecting properties of the “Mikadez” layer dryer. Sci. Tech. Anim. Husb. Nat. Management. 2021; 7: 63–67. DOI: 10.31890/vttp.2021.07.10. (in Ukrainian)
13. Montagna MT, Triggiano F, Barbuti G, Bartolomeo N, De Giglio O, Diella G, Lopuzzo M, Rutigliano S, Serio G, Caggiano G. Study on the in vitro activity of five disinfectants against nosocomial bacteria. J. Environ. Res. Public Health. 2019; 16 (11): 1895. DOI: 10.3390/ijerph16111895.
14. Moshynets OV, Spiers AJ. Viewing biofilms within the larger context of bacterial aggregations. In: Microbial Biofilms — Importance and Applications. Ed. by D Dhanasekaran, N Thajuddin. 2016: 3–22. DOI: 10.5772/62912.
15. Nechyporenko OL, Berezovskyi AV, Shkromada OI, Ulko LH, Nychyk SA. Assessment of ADG disinfectant cumulative and metabolic rate effect in laboratory animals. Biotechnol. 2020; 36: 128–137. DOI: 10.31073/vet_biotech36-13. (in Ukrainian)
16. Opryshko VI, Prokhach AV, Prokhach AV, Kurt-Akhmetova HS. Amazing nearby. Effective and safe protection against nosocomial infections. Surg. Perinatal Med. 2021; 11 (1/39): 73–76. DOI: 10.24061/2413-4260.XI.1.39.2021.10. (in Ukrainian)
17. Paliy AP, Ishchenko KV, Dubin RA. Effectiveness of aldehyde disinfectant against the causative agents of tuberculosis in domestic animals and birds. J. Ecol. 2018; 8 (1): 845–850. DOI: 10.15421/2018_283. (in Ukrainian)
18. Paliy AP, Ishchenko KV, Marchenko MV, Dubin RA. Effectiveness of aldehyde disinfectant against the causative agents of tuberculosis in domestic animals and birds. J. Ecol. 2018; 8 (1): 845–850. DOI: 10.15421/2018_283. (in Ukrainian)
19. Paliy A, Zavgorodniy A, Stegniy B, Gerilovych A. A study of the efficiency of modern domestic disinfectants in the system of TB control activities. Agricultural Science and Practice. 2015; 2 (2): 26–31. DOI: 10.15407/agrisp2.02.026. (in Ukrainian)
20. Ponomarenko GV, Kovalenko VL, Ponomarenko OV, Balackiy YO. Effect of microbicide on lactic acid and metal nanoparticles on laboratory animals. J. Ecol. 2017; 7 (4): 482–485. DOI: 10.15421/2017_148. (in Ukrainian)
21. Pustovit NA, Pinchuk NH. The use of test lenses in the disinfection systems in the fig against campylobacterios. NUBIP Rep. 2019; 78 (2): 75–85. DOI: 10.14738/dopovidi2019.02.021. (in Ukrainian)
22. Soto E, Abdelrazek SMR, Basbas PJ, Duignan PJ, Rios C, Byrne BA. Environmental persistence and disinfectant susceptibility of Klebsiella pneumoniae recovered from pinnipeds stranded on the California Coast. Microbiol. 2019; 241: 108554. DOI: 10.1016/j.vetmic.2019.108554.