THE INFECTIOUS ACTIVITY AND ANTIGENIC PROPERTIES STUDYING OF THE TESCHEN DISEASE VIRUS ISOLATES FROM DOMESTIC PIGS AND WILD BOARS IN UKRAINE
N.P. Sytiuk
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Institute of Veterinary Medicine NAAS of Ukraine, 03151, Donetska st., 30, Kiev, Ukraine
The data on the monitoring of two isolates of Teschen disease virus in the territory of Ukraine: «Shevchenkovo-05» isolated in 2005 from sow pathological material with clinical signs of disease and «Novoazovs'k 2013» isolated from wild boar killed in hunting season of 2013 year were presented in the title. The article described the clinical signs (fever up to 41,4˚C, malaise, anorexia, discoordination, hyperesthesia, twitching muscles of the forelimbs, shortness of breath, cyanosis of the mucosa, ears and snout skin) and pathological manifestation (redness and swelling of the brain tissue, nasal septa cyanosis, overflow the bladder) in the course of Teschen disease in pigs. The data for the detection rating of the causative agent in biological material samples obtained from pigs (the brain tissue) and wild boar (rectal swabs specimens) were presented in the title. It was presented the peculiarities of the isolation of causative agent with using of the primary display test into the PK cell line by detecting apoptosis of the cells in the four consecutive passages. The identification of the virus isolates in virus-containing suspension threatened with chloroform were used for the determination of an infectious activity. In order to identify the Teschen disease virus and its antigenic affinity was performed with selected virus-containing culture medium by the terminal dilutions where the cytopathic effect of the virus was detected and it was performed the 3 (three) successive passages in SKES cell line culture. It was determined the terms of cytopathic effect manifestation and the antigenic activity of virus isolates. Using cross-neutralization tests it was determined the neutralization index and antigenic affinity index toward Teschen disease virus reference strain «Perechynskyy 642». Using virologic and serologic tests it was determined that the isolates are derivates of Teschen disease virus.
Keywords: WILD AND DOMESTIC PIGS, THE VIRUS TESCHEN DISEASE, BIOLOGICAL MATERIAL, MONITORING, INFECTIOUS ACTIVITY, ANTIGENIC PROPERTIES, IDENTIFICATION
