CHOLINEESTERASE ACTIVITY AND ISOFORM CONTENT IN BREEDER SPERM
N. Nasedkina, D. Ostapiv, I. Jaremchuk, S. Kornyat
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Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine
Specific (acetylcholinesterase; AChE) and nonspecific (butyrylcholinesterase; nChE) cholinesterase activity and AChE isoform content in breeder sperm were studied. It is set, that boar sperm characterizes by low activity of AChE (16.3±1.44 nmol/min×mg protein) and nChE (6.0±0.94 nmol/min×mg protein), ram has a high values (67.7±3.21 and 25.0±2.05 nmol/min×mg protein), bull semen indicators occupy an intermediate position (AChE — 48.3±8.72 and nChE — 15.7±2.37 nmol/min×mg protein). It is found, that main part of AChE activity in breeder ejaculates falls on sperm plasma (59.5–81.6 %), and nChE — on bull and ram spermatozoa (74.8–93.6 %) and on sperm plasma — in boar (71.7 %). Bull and ram ejaculates characterizes by 5–6 main AChE isoforms and by 3–4 minor, and boar, correspondingly, 3–4 and 2–3. Quantitatively for boar ejaculates, as in plasma and bull sperm isoform content lowers with the speed migration increase of enzyme proteins in 7.5 % polyacrilamide gel. On the contrary, in bull sperm plasma and spermatozoa almost 1/3 of isoform content is AChE4 (29.3–34.1 %), lesser (10.3–18.0 %) — АChЕ3, АCЕ5 and АChЕ6 and the least is (1.9–6.6 %) АChЕ1, АChЕ2 and АChЕ7. Ram ejaculates and sperm plasma characterizes by higher content of АChЕ4 (30.9–33.1 %), lesser АChЕ5 (18.1–25.9) and the least — АChЕ7 (6.9–7.5 %). It was found that, AChE quantity and isoform content in sperm are caused by individual features, feeding and intensity of breeder usage.
Keywords: ACETYLCHOLINESTERASE, NONSPECIFIC CHOLINESTERASE, ISOFORMS, SPERM, SIRES
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