LIPIDS OF SHEEP’S SERUM BY EXPERIMENTAL INFECTION OF BOVINE LEUCOSIS VIRUS
L. M. Ishchenko, S. D. Melnychuk, V. D. Ishchenko, S. V. Sysolatin, V. G. Spyrydonov
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National University of Life and Environmental Sciences of Ukraine, Heroyiv Oborony st., 15, Kyiv-41, 03041, Ukraine
Воvine leukemia is a chronic disease caused by a retrovirus called BLV (Bovine Leukemia Virus). The feature of the pathogenesis of bovine leucosis virus is the introduction of cDNA into the genome of infected animal’s lymphocytes, which causes long latency period of the disease.
Experimental infection of sheep bovine leukemia virus has been conducted. Infection was performed by intramuscular injection of 1 cm3 of blood from positive leukemia cow. Indicators of white blood and lipid composition of sheep's serum were analyzed at the stage of the proviral DNA (9 days after infection) and at the stage of detection of antibodies against BLV (70 days after infection). It was shown that at the stage of provirus in the blood of infected animals the number of segmented neutrophils increased and lymphocyte level decreased versus control animals. And at the stage of the serological response the number of lymphocytes increased and the level of segmented neutrophils decreased. While studying the lipid composition of the serum of experimental group of animals at the stage of the proviral DNA, it was found out that serum contained more cholesteryl esters. And at the stage of antibodies detection against BLV, it contained more total phospholipids and the ratio of total cholesteryl to phospholipids decreased. Fatty acid composition of sheep’s serum at the stage of the proviral DNA was characterized by increase of fatty acid C17:0 and decrease of arachidonic acid (C20:4) level, and at the stage of production of antibodies by decrease of arachidonic acid (C20:4) amount and increase of linoleic acid (C18:2) in comparison with control group of animals. The decrease of arachidonic acid in both stages of infection in our view associated with the synthesis of prostaglandin E2, which affects the regulation of the expression of bovine leucosis virus.
Keywords:BOVINE LEUKEMIA, STAGES OF LEUKEMIA, SHEEP, EXPERIMENTAL INFECTION, PROVIRUS, MORPHOLOGICAL PARAMETERS OF BLOOD, LIPIDS, FATTY ACIDS
