PATHOMORPHOLOGICAL CHANGES IN CHICKENS CAUSED BYASCARIDIA GALLI
M. Holubtsova
vetparazytologia@gmail.com
Lviv National University of Veterinary Medicine and Biotechnologies named afterS.Z.Gzhytskyj,
50Pekarska str., Lviv 79010, Ukraine
This work displays the results of pathological studies and histopathological changes in tissues of digestive tract and liver in chickens infested by Heterakis. The autopsy was performed by the standard method. As pathoanatomical material we used pieces of the large intestine and liver of chickens which died as a result of heterakis infestation. The material was fixed in 10 % neutral formalin solution, followed by dehydration in ethanol, sealed in chloroform, chloroformparaffin and fill in paraffin, which was kept for 2 hours. Sections were prepared at sledge microtome, followed by painting them with hematoxylin and eosin. Manifestation of patoloanatomical changes features largely dependent on the intensity of infestation. For the macroscopic study characteristic changes found in the cecum and liver. With the opening of the blind gut mucosa swollen, red, covered with nodules that are caused by the larvae of heterakis. For histology blind guts installed congestion and mucosal edema, impaired epithelial integrity and tissues that are below, as well as atrophy and deformation crypts. On examination of the liver showed heterogeneous dark cherry coloration, against which showed gray, different sizes rounded cells. For chicken liver histology set necrobiotic-dystrophic processes in hepatocytes. Hepatocytes in a state of malnutrition. Around the crypt showed clusters of micro-macrophage cell system. The results showed that the major patolohomorfological changes localized in cecum and liver and were characterized by catarrhal-degenerative and destructive processes.
Keywords: CHICKENS, HETERAKIS, РATHOMORPHOLOGICAL CHANGES, HISTOLOGICAL CHANGES, LARGE INTESTINE, LIVER