POLYMORPHISM OF MICROSATELLITE DNA SEQUENCES IN DOGS RUSSIAN TOY TERRIER AND GERMAN DOG

Bìol. Tvarin, 2016, vol. 18, no. 1, pp. 27–32

https://doi.org/10.15407/animbiol18.01.027

POLYMORPHISM OF MICROSATELLITE DNA SEQUENCES IN DOGS RUSSIAN TOY TERRIER AND GERMAN DOG

V. Dzitsiuk¹, S. Kruhlyk², V. Spyrydonov²

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¹The Institute of Animals Breeding and Genetics NAAS,
Chubynske, Boryspil district, Kyiv region, 08321, Ukraine

²Ukrainian Laboratory of Quality and Safety of Agricultural Products,
National University of Life and Environmental Sciences of Ukraine,
Chabany, Kyiv-Sviatoshyn district, Kyiv region, 08162, Ukraine

The level of genetic polymorphism of five micro-satellite loci for the German Dog and the Russian Toy-Terrier dog breeds which are bred in Ukraine is researched. The study is aimed to value the micro-satellite panel for checking and identifying the relationship of domestic dog breeds. The five micro-satellite loci were used for the genetic analysis: PEZ1, PEZ6, PEZ8, recommended by the American Kennel Club, and FHC 2010, FHC2054, recommended by ISAG in order to genotype the dogs.

The existence of individual and breed differences for the German Dog and Russian Toy-Terrier breeds is found. It is established that the average number of alleles per locus is 8.2 for the sample of Russian Toy-Terriers and 5.8 for the German Dogs. The parameters of heterozygosis were calculated. The Hobs and Hexp indices show that in general both groups tend to homozygotisation — the actual heterozygosis is lower than expected. The calculated polymorphism (PIC) indices display a high level of polymorphisms for both studied samples. The PIC Index ranged from 0.580 per locus FHC2010 to 0.866 per locus PEZ6 for the Russian Toy-Terrier group and from 0.735 per locus PEZ1 to 0.874 per locus PEZ8 for the German Dogs. The average group RIS values indicate a higher polymorphism for the German Dog micro-population (0.810 in average).

The rare alleles were discovered as well as the polymorphism was noted for each locus in the investigated samples of animals. The exclusions of coincidental match of alleles at 99.8 % provide the opportunity for using the resulting population-genetic information to confirm the origin, individual identification and breed certification of the dogs.

Keywords: DOG, DNA-MARKERS, POLYMORPHISM OF MICROSATELLITE, TOY-TERRIER, GERMAN DOG, FLLELES, LOCI

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