CARBOHYDRATE METABOLISM IN BLOOD AND LIVER OF RATS WITH EXPERIMENTAL DIABETES AND ITS CORRECTION BY ZINC CITRATE

Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 46–52

http://dx.doi.org/10.15407/animbiol18.03.046

CARBOHYDRATE METABOLISM IN BLOOD AND LIVER OF RATS WITH EXPERIMENTAL DIABETES AND ITS CORRECTION BY ZINC CITRATE

R. Ya. Iskra, O. M. Slivinska, H. V. Klymets

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Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

The influence on zinc citrate in a quantity 20 and 50 mg Zn/kg of body weight on the indexes of the carbohydrate metabolism in rats’ blood and liver during the streptozotocin-induced diabetes was investigated. Experiments were conducted on white laboratory rats which were divided into four groups: I — the control group, II, III, IV — research groups. An experimental diabetes mellitus (EDM) was induced in all animals of research groups by intraperitoneal injection of Streptozotocin in 45 mg/kg body weight dosage, after 24-hour fasting. Rats from the group II with the EDM recieved only basic ration, while zinc citrate solution was added to the ration of animals from groups III and IV for four weeks in 20 and 50 mg Zn/kg body weight dosage.

It was determined during the research that blood levels of glucose, glycosylated hemoglobin, lactate and the activity of lactate dehydrogenase in erythrocytes and liver tissue increased but the blood level of pyrivate and the activity of glucose-6-phosphate dehydrogenase in erythrocytes and liver tissue decreased. The normalization of these indexes occurred under the condition of zinc citrate addition to the rats’ diet in 20 and 50 mg Zn/kg body weight dosage. This shows the possitive effect of Zinc on rats’ carbohydrate metabolism in a state of experimentally-induced diabetes. The recieved results may indicate on the possibility of the prevention of this disease in animals and people with the administration of zinc citrate.

Keywords: RATS, CARBOHYDRATE METABOLISM, ZINC CITRATE, EXPERIMENTAL DIABETES MELLITUS

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