INFLUENCE OF LOW DOSES OF TAURINE ON ANTIOXIDANT DEFENSE AND LIPOPROTEIN COMPOSITION IN RAT BLOOD

Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 94–100

http://doi.org/10.15407/animbiol19.02.094

INFLUENCE OF LOW DOSES OF TAURINE ON ANTIOXIDANT DEFENSE AND LIPOPROTEIN COMPOSITION IN RAT BLOOD

R. Ostapiv^1,2, Chr. Skyba¹, V. Manko¹

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¹Ivan Franko National University of Lviv,
4, Hrushevskyi str., Lviv 79005, Ukraine

²SCIVP of Veterinary Medical Products and Feed Additives,
11, Donetska str., Lviv 79019, Ukraine

The aim of our work was to investigate the influence of long-term peroral taurine injection on antioxidant defense and lipoprotein composition in rat blood. Studies were conducted on male 4 months old Wistar rats (n=16) with weight 140–160 g. Animals were divided into four groups (4 rats in each): control (animals were daily during 28 days injected drinking water in esophagus), and three experimental groups that were injected 5 (1^st experimental group), 10 (2^nd experimental group) and 20 mg (3^rd experimental group) of taurine per kg of body weight. On the 29^th day of experiment rats were decapitated, and in blood activity of enzymes and specific izoenzymes of antioxidant defense (superoxide dismutase, glutathione peroxidase, catalase), content of TBA-active products, lipoprotein blood and soluble proteins composition were determined.

It was registered that long term per oral taurine injection does not influence the activity of antioxidant defense enzymes, though TBA-active products content decreases and activity of isoenzymes change. Activity of SOD1 in blood of 2^nd experimental group animals increases in twofold, and in 3^rd experimental group SOD3 dominates. It was determined that after injection of 10 and 20 mg/kg taurine the activity of CAT1, CAT2 and GPO3 increased 1.5–4 times. In blood of animals in 1^st and 3^rd experimental groups the content of albumins increases, but the content of prealbumins decreases. That may point on intensification of metabolism. In rats of 2^nd experimental group the content of γ- та β-globulins increases, though the content of prealbumins decreases. In blood of 1^stexperimental group the content of lipoprotein of very high and low density increases, herewith content of chylomicrons decreases. On the contrary, in rats of 3^rd experimental group chylomicrons content increases but the content of lipoproteins with very high density decreases.

Keywords: ANTIOXIDANT DEFENSE, BLOOD, RATS, SUPEROXIDE DISMUTASE, GLUTATHIONE PEROXIDASE, CATALASE, TBA-ACTIVE PRODUCTS, LIPOPROTEINS, BLOOD SOLUBLE PROTEINS

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