HISTOMORPHOLOGY OF ORGANS AND ACTIVITY OF SOME ENZYMES IN MICE AFTER IMMUNIZATION WITH POLYMER BASED ON ACRYLIC ACID OR ALUMINUM HYDROXIDE AS ADJUVANTS

Bìol. Tvarin, 2018, volume 20, issue 1, pp. 54–59

http://doi.org/10.15407/animbiol20.01.054

HISTOMORPHOLOGY OF ORGANS AND ACTIVITY OF SOME ENZYMES IN MICE AFTER IMMUNIZATION WITH POLYMER BASED ON ACRYLIC ACID OR ALUMINUM HYDROXIDE AS ADJUVANTS

M. R. Kozak¹, Yu.V. Martyn¹, A. V. Oliynyk¹, N. V. Kuzmina¹, D. D. Ostapiv¹, O. L. Pajuk²,
N. E. Mitina², O. S. Zaichenko², V. V. Vlizlo¹

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¹Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

²National University “Lviv Polytechnic”,
2 Sviatoho Yura sq., Lviv 79013, Ukraine

Vaccination is the best biomedical approach in avoiding diseases. Proteins and peptides purified from microorganisms or synthesized chemically are weakly antigenic and need adjuvant to provide strong immune responses. Many substances with adjuvant properties have been discovered, however, only aluminum compounds stay traditionally in clinical use. The aim of this study was to evaluate biological influence on mice after immunization with a polymer of acrylic acid with adjuvant properties.

The polymer was synthesized on the basis of glycidyl methacrylate, acrylic acid, triethylene glycol methacrylate and butyl acrylate. Histomorphology of the liver, kidney and spleen of white mice after subcutaneous administration of polymer or aluminum hydroxide was investigated. It has been established that the polymer and aluminum hydroxide did not cause changes in parenchymal organs of white mice. The structure of organs was preserved, no pathological changes were revealed.The activities of antioxidant enzymes — superoxide dismutase (SOD), catalase and glutation reductase (GP) at the application of adjuvants were studied. Immunization of mice with aluminum hydroxide showed an increase in the activity of SOD in the kidneys by 1.5 times (P<0.01), catalase in liver on 8 % (P<0.05), and GP in kidney on 30.6 % (P<0.001). Polymer based on acrylic acid caused a decrease of SOD activity in the liver by 1.78 times (P<0.01), and an increase of catalase activity on 10.5 % (P<0.05). At the same time, the activity of antioxidant enzymes in the spleen and kidneys of animals after the injection of the polymer did not differ from the control group.

Keywords: ORGANS OF MICE, POLYMER, ALUMINUM HYDROXIDE, ENZYME ACTIVITY

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