Bìol. Tvarin, 2018, volume 20, issue 1, pp. 23–27
THE INFLUENCE OF PHOSPHOLIPID-CONTAINING PREPARATIONS ON THE LEVEL OF IMMUNOGLOBULIN M IN THE SERUM OF BLOOD OF CALVES DURING THE PERIOD OF FORMATION OF COLOSTRAL IMMUNITY
S. I. Golopura, B. V. Popadiuk, M. I. Tsvilihovsky
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National university of life and environmental sciences of Ukraine,
16 Polkovnyka Potekhina str., Kyiv 03041, Ukraine
The results of application of preparation “Membranostabil” and phospholipid-bilayer macrocapsules on the base of soy-bean lecithin developed by us with the aim of correction of indexes of content of immunoglobulin M in the blood of newborn calves during formation of colostral immunity is shown. The indexes of content of immunoglobulin M in the serum of blood of the newborn calves in dynamic were investigated — from birth till the 11th day of life.
The research was conducted on the three of groups of calves (the control one and two research) of Ukrainian black and white milk breed with drawing of blood samples from the jugular vein. Immunoglobulins M were examined by gel electrophoresis in a thin layer of a polyacrylamide gel. Quantitative estimation of protein fractions was carried out by scanning of the electrophoregrams with subsequent reconstructing of them graphically and calculating by relative units or area using a computer program.
The results have proved that the preparation “Membranostabil” and phospholipid-bilayer macrocapsules based on soy-bean lecithin activate transport of immunoglobulins in small intestine and promote veracious increasing of the content of immunoglobulin M compared to the control group. The content of immunoglobulin M in the serum of newborn calves in both of the research groups at the age of 6 hours credibly increased and was higher during the whole period of the research except for calves of the 1st research group at the age of 7 days compared to the calves of the control group. The dynamic with comparative analysis of content of immunoglobulin M in the serum of blood between different groups of calves of control and research groups is shown. The increasing of the content of immunoglobulin M in blood of newborn calves prevents development of digestive disorders in these animals.
Keywords: COLOSTRAL IMMUNITY, СOLOSTRUM, BLOOD SERUM, IMMUNOGLOBULIN M, NEWBORN CALVES
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