Bìol. Tvarin, 2018, volume 20, issue 1, pp. 9–15
INVESTIGATION OF THE ANTIBACTERIAL ACTION OF MODIFIED HETEROCYCLIC COMPOUNDS OF SUBSTITUTED ACRIDONES AGAINST STAPHYLOCOCCUS AUREUS
M. M. Babkina1, O. V. Vasylchenko2, O. M. Deriabin3, A. A. Tarasov4, A. M. Golovko3, L. G. Palchykovska2
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1Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine
38 V. Stus str., Lviv 79034, Ukraine
2Institute of Molecular Biology and Genetics NAS of Ukraine,
150 Akademika Zabolotnoho str., Kyiv 03680, Ukraine
150 Akademika Zabolotnoho str., Kyiv 03680, Ukraine
3State Scientific Control Institute of Biotechnology and States of Microorganisms,
30 Donetska str., Kyiv 03151, Ukraine
30 Donetska str., Kyiv 03151, Ukraine
4Institute of Veterinary Medicine NAAS,
30 Donetska str., Kyiv 03151, Ukraine
30 Donetska str., Kyiv 03151, Ukraine
The results of the study of antibacterial activity and determination of minimum inhibitory concentration substituted acridones class by microdilution method against a Staphylococcus aureus are described in the article. The antibacterial activity of the substituted acridones class was investigated by disk diffusion method. An antibacterial activity of substituted acridones class to museum strain to Staphylococcus aureus was established. The presence of the susceptibility of Staphylococcus aureus to the tested compounds has been revealed.
In this research we used the substituted acridones class compounds. We chose fourteen the most perspective substances with some difference due to the position of radical groups and it composition recording the results of the research.
As the result of investigation the minimal inhibitory concentration and growth of inhibition zones of substituted acridones class substances was identified. The minimal inhibitory concentration was detected at range from 0.41±0.004 mg/cm3 to 0.0041±0.00005 mg/cm3 and growth zone diameter was from 8.2±0.4 mm to 24.1±0.31 mm.
The most active compounds tested of substance regards to Staphylococcus aureus were ODI-90 (2,5-difluoro-9-oxo-N-pyridin-4-yl-9,10-dihydroacridine-4-carboxamide) with a minimum inhibitory concentration 0.0041±0.00005 mg/cm3 and the diameter of growth inhibition zone 24.1±0,31mm. Compounds ODI-76, ODI-77, ODI-80, ODI-85 had the minimum inhibitory concentration 0.041 mg/cm3 and the diameter of growth inhibition zone from 20.8±0.34 mm to 21.2±0.4 mm. The compounds ODI-64, ODI-65, ODI-68, ODI-71, ODI-72, ODI-73, ODI-84, ODI-88, ODI-89 which differ by their nature and position of the radicals demonstrated low level of antibacterial activity with a minimal inhibitor concentration. The diameter of the zones of growth inhibition for these compounds was from 8.2±0.4 mm to 14.0±0.36 mm.
The compounds ODI-61, ODI-62, ODI-63, ODI-66, ODI-67, ODI-69, ODI-70, ODI-74, ODI-75, ODI-78, ODI-79, ODI-81, ODI-82, ODI-83, ODI-86, ODI-87 did not demonstrate any activity against Staphylococcus aureus.
Keywords: MICROORGANISM, STAPHYLOCOCCUS AUREUS, MINIMAL INHIBITORY CONCENTRATION, ANTIBACTERIAL PROPERTIES, SUBSTITUTED ACRIDONES
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