The Animal Biology

EFFECT OF ETHYLTHIOSULFАNYLATE AND CHROME(VI) ON THE PRO/ANTIOXIDANT SYSTEM IN RATS BLOOD

B. Kotyk, R. Iskra, O. Sushko, O. Slivinska, G. Klymets, O. Buchko, A. Pylypets, V. Pryimych

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Institute of Animal Biology NAAS,
38 V. S Stus str., Lviv 79034, Ukraine

The purpose of the research was to investigate the effect of ethylthiosulfanylate as a synthetic analogue of natural esters of thiosulfonic acids on the state of the antioxidant system in the blood of laboratory rats exposed to Cr(VI). Animals were divided into 7 groups. Animals of group I were intraperitoneally injected 150 μl of saline solution daily for 7 days. Animals of groups III and IV were intraperitoneally administered K₂Cr₂O₇ in a dose of 2.5 mg Cr(VI)/kg body weight daily for 7 (group III) and 14 days (group IV). Rats of group II received intragastric injection of 1000 μl of oil daily for 14 days, and then animals were intraperitoneally injected 150 μl of physiological solution daily for 7 days. Rats of group V were intragastrically injected with ethylthiosulfanylate oily solution at a rate of 100 mg/kg of body weight daily for 14 days, and then animals were intraperitoneally injected 150 μl of physiological solution daily for 7 days. Rats of groups VI and VII were intragastrically administered with ethylthiosulfanylate oily solution at a rate of 100 mg/kg of body weight daily for 14 days, than animals were intraperitoneally injected K₂Cr₂O₇ in a dose of 2,5 mg Cr(VI)/kg body weight daily for 7 (group VI) and 14 days (group VII). After the decapitation of the animals during thiopental anaesthesia, a blood samples were taken. It has been found that the introduction of bichromate of potassium in the blood of rats in groups III and IV shows an increase in the content of oxidative stress. Preliminary intragastric administration of ethylthiosulfanylate partially reduced the level of growth of hydroperoxides of lipids and thiobarbituric acid TBA-active products caused by the action of Cr(VI) in groups VI and VII and did not affect the level of carbonyl groups of proteins products, in particular lipid hydroperoxides, thiobarbituric acid (TBA-active products and carbonyl groups of proteins. The work of the glutathione link of antioxidant protection under the influence of Cr(VI) was partially activated in group III, but suppressed in group IV. Ethylthiosulfanylate separately (group V) and in combination with Cr(VI) (groups VІ and VІІ) caused activation of glutathione reductase and increased GSN concentration in erythrocytes of rats compared with group ІІ. Superoxide dismutase and catalase activity of rat erythrocytes were increased by the action of Cr(VI) in groups III and IV in comparison with group I. However, with the effect of ethylthiosulfanylate in Cr(VI) complex, superoxide dismutase activity was increased in VII and catalase activity in VI and VII groups decreased, compared to group II.

Keywords: RATS, ANTIOXIDANT SYSTEM, OXIDATIVE STRESS, ETHYLTHIOSULFАNYLATE, BICHROMATE OF POTASSIUM, FREE RADICALS

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