The Animal Biology

Bìol. Tvarin, 2015, volume 17, issue 3, pp. 21–28

ELUCIDATION OF PHARMACO-TOXICOLOGICAL PROPERTIES AND ESTABLISHING OPTIMAL THERAPEUTIC DOSE OF “TIMTIL”

A. V. Berezovsky, L. G. Ulko, V. V. Sencha

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Sumy National Agrarian University,
G. Kondratiev str., 160, Sumy, 40021, Ukraine

The paper presents the results of studies of pharmacy-toxicological properties and the optimal therapeutic dose of “TimTil”. Results of studies found that possible changes in the behavior of the experimental animals were not observed when using the drug “TimTil” within 21 days. With the administration of the drug in 1/10 DL₅₀ the motor activity of animals decreased and they showed signs of depression. Emotional and behavioral responses of white mice in the experimental and control gruops were not significantly different. We noted a slight increase in body weight of experimental and control animals throughout the experiment. The low percentage of increase in the total mass was observed in the third group of mice receiving the drug “TimTil” in a dose 1/10 DL₅₀. Analysis of hematological parameters indicates insignificant decrease in hemoglobin in the blood of animals of the third group. Contents of erythrocytes and leukocytes in the blood of experimental and control animals were not significantly different. An increase of the number of neutrophilеs and lymphocytes decreased with increasing doses of study drug.

During the conjunctive sample it was revealed that “TimTil” shows no irritant action on the mucous membrane of the eyes of experimental animals. During the study period all animals remained active without reducing the weight and the visible signs of deviation from the normal behavioral responses.

The results indicate that the drug “TimTil” has no allergenic and local irritant effect. According to data obtained by the study of the local irritant action of the drug “TimTil” the single and long-term application to the skin of laboratory animals stated the absence of on-site application of the inflammatory reaction, itching, ulceration, and alopecia, skinfold thickness did not change.

At a dose of 0.1 ml/kg body weight of the drug “TimTil” is effective in the treatment of white mice infected by cultures S. pyogenes, S. aureus, P. vulgaris, E. coli, F. necrophorum, C. perfringens.

Keywords: DRUG “TIMTIL”, PHARMACO-TOXICOLOGICAL PROPERTIES, TOXICITY, WHITE MICE, CLINICAL STATUS, HEMATOLOGICAL PARAMETERS, THE THERAPEUTIC DOSE

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