HISTOLOGICAL STRUCTURE OF SEPARATE INTERNAL ORGANS OF PIGLETS AT APPLICATION OF PROBIOTIC FEED ADDITIVE
M. I. Zhyla
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State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Donetska str., 11, Lviv, 79019, Ukraine
The aim of the test was to study the influence of probiotic feed additive in doses recommended by the producer and duration of application on the histological structure of separate internal organs of piglets. Theproduct was added to feed in different concentrations: the first group was given probiotic Probion-forte produced by WooGene (Korea) in dose of 1 g/kg (10 weeks); the second one — Probion-forte in dose of 1g/kg (6 weeks) and 0.5 g/kg (4 weeks); the third one was given probiotic Bio Plus 2B produced by Biochem (Germany) in dose of 0.4 g/kg for 10 weeks; and the fourth one was a control group. Each group contained 30 units. On the 42nd and on the 70th day 10 units form the groups were taken for pathomorphologic tests.
The tests showed that application of probiotic Probion-forte to piglets in form of feed additive for the period of 10 weeks after weaning promotes better assimilation of compound feed and gradual weight gain in comparison with the control group.
The microscope study of thymus, spleen inferior mesentericlymphnodes,intestine and liver showed that typical histological structure in all treated groups was preserved. The morphometric study showed the increase of villus height in duodenum of piglets fed by probiotic. The thymus weight of piglets in the experimental group on the 70th day of test was significantly bigger than in the control one, lobular structure was well expressed, parts of polygonal form, allocation of cortex and medulla were preserved during the test period. The thymocytes were densely situated. In the peripheral organs of immune system lymphoid tissue is sufficiently presented, lymphoidfollicles of spleen and inferior mesenteric lymph nodes are well formed, of small and average sizes, some of them are formed with reactive centres, cell population is high enough. While in the control group on the 70th day of test in thymus we observed decrease and focal devastation of cortex, lengthening of thymus parts, thickening of interlobular connective tissue. In spleen and lymph nodes we observed decrease of sizes and cell devastation of lymphoid follicles.
The efficacy and expediency of application were acknowledged in the experimental groups. The most significant difference in indices was observed in piglets fed by Probion-forte during the period of 10 weeks in dose of 1,0g/kg of feed.
Keywords: PROBIOTICS, FEED ADDITIVE, MORPHOLOGY, PIGLETS, HISTOSTRUCTURE, INTERNAL ORGANS, THYMOCYTES, LYMPHOID FOLLICLES, VILLUS HEIGHT
