METHOD FOR DETERMINING VIRULENCE OF PATHOGENS OF TINEA ANIMALS
A. M. Volkov
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National University of Life and Environmental Sciences of Ukraine,
Heroyiv Oborony str., 15, Kyiv–41, 03041, Ukraine
The paper presents a method for determining the virulence of pathogenic dermatomitsetes isolated from sick animals. The method is based on the use of the macerated skin young immature animals (mice, rats, guinea pigs, puppies, cats, rabbits, calves and so on) that gives an opportunity to diagnose dermatomycoses caused dermatomycetes genera Microsporum and Trichophyton. Method for determining the virulence of pathogens dermatomycoses animals is simple in execution, available for practice, effective and promising for the characterization of microorganisms that can be used as vaccines and control strains in the production of immunobiological preparations for specific prevention dermatomycoses animals. A method for determining the virulence of pathogens dermatomycoses animals using skin young immature animals allows to estimate the degree of virulence dermatomycetes after repeated recurrence of pathological focus. The method allows to share dermatomycetes by the nature of the pathological process in the skin and the timing of the flow of a skin reaction on the degree of virulence in four groups according to the degree of virulence: highly virulent, srednevirulentnye, slabovirulentnyh, avirulent. Method for determination of virulence dermatomycetes can be used in the selection process of production and control strains — producers for effective protective antigens dermatomitsetes to create a means of specific prophylaxis. Comparative results of applying the method of assessment of the virulence of pathogenic dermatomycetes on different kinds of immature animals have shown that the degree of the response differs depending on the type of pathogen. This should be considered for the selection of an appropriate animal species as a test object in each case, in particular in monitoring the effectiveness of vaccines.
Keywords: VIRULENCE, YOUNG ANIMALS, DERMATOMYCOSESE, STRAINS, MICE, RATS, GUINEA PIGS, KITTENS, PUPPIES, RABBITS, CALVES
