Detoxification processes in the cows fed nickel citrate supplement at late pregnancy and first months of lactation

O. I. Koleschuk¹, I. I. Kovalchuk², M. M. Tsap², M. M. Khomyn²

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¹Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv,
50 Pekarska str., Lviv, 79010, Ukraine

²Institute of Animal Biology NAAS,
38 V. Stus str., Lviv, 79034, Ukraine

The article presents experimental data on the effect of nickel citrate obtained using nanotechnology on the biochemical parameters of cows’ blood. The animals were divided into 3 groups. Group I was the control one. The animals of the II and III experimental groups received a feed additive of nickel citrate in the amount of 0.1 and 0.3 mg/kg of dry matter of the diet daily during the ninth month of lactation and the first two months after calving. It was found that the addition of both doses of nickel citrate to the transition cows diet contributed to positive changes in some biochemical parameters. A decrease in the content of lipid hydroperoxides, TBA-active products, as well as phenolic compounds was revealed. It should be noted that supplementation cows with nickel citrate in the first month after calving led to a significant increase in the content of lipid hydroperoxides by 15.1% in the third experimental group (P<0.01) against decrease in the level of TBARS by 14.8% compared with the control group (P<0.05). Feeding cows of nickel citrate in the amount of 0.1 mg/kg of dry matter stimulated the binding of free phenols and increased the concentration of their conjugated compounds, particularly phenolglucuronides, in the blood of animals of experimental group II by 20.2% (P<0.05). Instead, the use of nickel citrate in the amount of 0.3 mg/kg of dry matter contributed to a more pronounced activation of detoxification function with increasing concentrations of phenolsulfates and phenolglucuronides in the blood of animals of experimental group III compared with animals of control group by 23.1 and 21.2% (P<0.05).

Key words: cows, nickel citrate, lipid peroxidation, lipid hydroperoxides, phenols

1. Bartosz G. Reactive oxygen species: destroyers or messengers? Biochemical Pharmacology, 2009; 77 (8): 1303–1315. https://doi.org/10.1016/j.bcp.2008.11.009
2. Brucka-Jastrzębska E, Protasowicki M. Elimination dynamics of nickel, administered by a single intraperitonial injection, in common carp, Cyprinus carpio L. Acta Ichthyol. Piscat. 2004; 34 (2): 181–192. https://doi.org/10.3750/AIP2004.34.2.06
3. Djordjević VB. Free radicals in cell biology. International Review of Cytology. 2004; 237: 57–89. https://doi.org/10.1016/S0074-7696(04)37002-6
4. Fedoruk RS, Kravtsiv RY. Physiological mechanisms of adaptation of animals to environmental conditions. Animal biology. 2003; 1 (1–2): 75–82. (in Ukrainian)
5. Forgács Z, Némethy Z, Révész C, Lázár P. Specific aminoacids moderate the effects on Ni²⁺ on the testosterone production of mouse Leydig cells in vitro. Toxicol. Environ. Health A. 2001; 62 (5): 349–358. https://doi.org/10.1080/152873901300018075
6. Hostynek JJ. Sensitization to Nickel: etiology, epidemiology, immune reactions, prevention, and therapy. Rev. Environ. Health. 2006; 21 (4): 253–280. https://doi.org/10.1515/REVEH.2006.21.4.253
7. Jadhav SH, Sarkar SN, Ram GC, Tripathi HC. Immunosuppressive effect of subchronic exposure to a mix ture of eight heavy metals, found as ground water contaminants in different areas of India, through drinking water in male rats. Arch. Environ. Contam. Toxicol. 2007; 53 (3): 450–458. https://doi.org/10.1007/s00244-006-0177-1
8. Kaplunenko VG, Kosinov NV, Polyakova DV. Getting new nutrients and biocidal nanomaterials using erosion-explosive dispersion of metals. Proceedings of the Materials Research and practical conference with international participation “Nanotechnology and nanomaterials in biology and medicine”. SibUPK, Novosibirsk. 2007: 134–137. (in Russian)
9. Khomyn MM, Fedoruk RS, Kropyvka SY. Biochemical processes in the cows and biological value of milk under the influence of citrate chromium, selenium, cobalt and zink. Bìol. Tvarin. 2015; 17 (1): 155–162. Available at: http://aminbiol.com.ua/index.php/archive/129-archive/bt-17-1-2015/1414 (in Ukrainian)
10. Murlooney SB, Hausinger RP. Nickel uptake and utilization by microorganisms. FEMS Microbiology Reviews. 2003; 27 (2–3): 239–261. https://doi.org/10.1016/S0168-6445(03)00042-1
11. Ponkalo LI. Intensity of peroxidation of lipids and activeness of glutathion system of antoixidant protection in calves and their calves under the influence of new immunotropic medication in the form of liposomal emulsion. Bìol. Tvarin. 2012; 14 (1): 551–556. Available at: http://aminbiol.com.ua/index.php/archive?catid=1:2013-02-15-09-09-13&id=175:2013-03-08-08-43-55 (in Ukrainian)
12. Sitar OV, Novitsky NV, Taran NY. Nanotechnology in modern agriculture. Physics of Living. 2010; 18: 113–116. https://doi.org/10.1177/110330880901800108 (in Ukrainian)
13. Valko M, Morris H, Cronin MTD. Metals, toxicity and oxidative stress. Curr. Med. Chem. 2005; 12 (10): 1161–1208. https://doi.org/10.2174/0929867053764635
14. Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory methods of investigation in biology, stock-breeding and veterinary medicine: a reference book. Lviv, Spolom, 2012: 764 p. (in Ukrainian)
15. Vlizlo VV, Kurtyak BM, Vudmaska IV, Vishchur OI, Petruk AP. Fat-soluble vitamins in veterinary medicine and animal husbandry. Lviv, Spolom. 2015: 436 p. (in Ukrainian)