Research on fungicidal effect of “Diolide” disinfectant

O. M. Chechet¹, V. L. Kovalenko²

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¹State Scientific and Research Institute for Laboratory Diagnostics and Veterinary and Sanitary Expertise,
12 Volynska str., Kyiv, 03151, Ukraine

²State Research and Control Institute for Biotechnology and Microorganisms Strains,
30 Donetska str., 03151, Kyiv, Ukraine

Nowadays it is important to develop new disinfectants for poultry which are simple in use, non-toxic if used in the presence of birds, with a broad bactericidal spectrum, not causing the resistance of microflora, maintaining the air environment, linking ammonia and deodorizing the air. One of the product effectiveness indicators comes from testing on micromycetes which are persistent on the indoor surfaces and require high fungicidal concentrations of active substances. Research results of the effect produced by the “Diolide” disinfectant with 42% sodium chlorite and 46% sodium chloride base showed fungicidal activity against the etalon strains of Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404. By serial digestion method using paper disks on Chapek’s medium and on the malt agar in the presence of the “Diolide” disinfectant the effective concentration of 0.1% was established. “Diolide” disinfectant at 0.1% concentration at 60 min. exposure completely disinfected test objects of wood, iron, brick and plaster which were contaminated with micromycetes. It was found that the effectiveness of “Diolide” disinfectant depends directly on the time of exposure and it is therefore necessary to adhere to these conditions in the production process.

Key words: micromycetes, disinfectant, test objects, test microorganisms, fungicidal effect

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