THE CORRELATION OF MOUSE OOCYTE MEIOTIC MATURATION WITH CULTURE CONDITIONS IN VITRO
I.V.Lobachova
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The Ivanov’s Institute of Animal Breeding in Steppe Regions «Ascania-Nova»
st. Chervonoarmiyska 1, Ascania-Nova, Charlynsky r., Kherson pr., 75230 Ukraine
It had been estimated the correlation of the index of in vitro meiotic maturation (M2) of oocyte of random crossed (C57BL×CBA)mouse with the following culture parameters: culture drop volume, concentrations of FSH, LH, estradiol, pyruvate, lactate, glucose, BSA, blood serum. Basis of culture medium was SOF added aminoacids, antibiotics, glutamine. Oocytes were cultured by isolated groups — oocytes surrounded cumulus and denuded oocytes. Analysis was carried with due regard for presence/absence serum in culture medium. M2 index and rate of correlation of M2 with the culture conditions had exhibited dependence with the start morphology of oocytes. Both in non-serum and serum culture variants the part of oocytes which chromosomes achieved M2 stage in the oocyte surrounded group was more that in the oocyte denuded group. M2 moderate (0,3<r<0,5)negatively correlated with the culture drop volume, moderate positively — with the pyruvate and glucose contents, weakly (r<0,3)— with concentration of gonadotropins and lactate content. Meiotic maturation of oocyte surrounded cumulus was significant (p<0,05) positively affected the contents of energetic components and serum, significant negatively — the culture drop volume and estradiol content; maturation of denuded oocytes was significant positively affected the contents of energetic components, significant negatively —the culture drop volume. It was discovered that morphological parameters of cumulus of oocytes matured in vitro with different rate correlate with M2 index and culture conditions and may be to use as additional criteria for estimation of maturation procedure quality.
Keywords: MOUSE, OOCYTE, MEIOTIC MATURATION, IN VITRO, DROP VOLUME, HORMONES, GLUCOSE, PYRUVATE, LACTATE, BSA, SERUM, CORRELATION
