ANALYSIS OF THE IMPACT OF CHRONIC LOW-DOSE IONIZING RADIATION ON THE MUS MUSCULUS LINE AF AND THEIR NEXT GENERATION
О. V. Fedorova, S. O. Kostenko
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National University of Life and Environmental Sciences of Ukraine
Kyiv, str. Heroyv Oborony, 12-B, korp.7-A, 03041, Ukraine
The accident that occurred at Chernobyl is one of the largest technological disasters in the history of mankind. As a result of the accident, were subjected to the influence of ionizing radiation in Ukraine, Russia and Belarus. Studies on the effects on living organisms of the Chernobyl accident have become particularly relevant in recent decades, as even small doses increase the likelihood of mutations.
In order to assess the impact of chronic low-dose ionizing radiation performed cytogenetic analysis of Mus musculus laboratory strain Af, and their children (F1). Conducted diagnostic radiation-induced genetic damage in the body, directly experienced the effects of the operation of chronic low-dose ionizing radiation, analyze the possibility of their transfer to the next generation of genetic instability.
Animals were housed one month under the action of chronic low-dose ionizing radiation (3,3±0,1 mcg / g), after which the animals within 10 days mated. Cytogenetic analysis was performed with the Mus musculus Chernobyl exclusion zone. Cytogenetic preparations were made from bone marrow, which we extracted from the femur of animals. In each animal analyzed at least 3000 cells.
It has been found that as a result of chronic exposure to ionizing radiation, animals an increase in the frequency of bone marrow cells with micronuclei (5,9 ± 0,96 ‰) compared to control (4,20 ± 0,16 ‰). The children of irradiated parents found an increase in the mitotic index (4,5±0,33 ‰) compared with controls (3,1±0,21 ‰). The results of various authors about the transgenerational transmission of genomic instability are contradictory, so it is important to conduct long-term monitoring of the vital activity of organisms and their descendants in the contaminated area.
Keywords: MICRONUCLEUS, CHRONIC LOW-DOSE IONIZING RADIATION, MITOTIC INDEX, CHERNOBYL, GENE, TRANSGENERATIONAL TRANSFER, CYTOGENETIC MONITORING
