ACTIVITES OF ENZYMES ANTIOXIDANT DEFENCE IN TISSUES OF PIGLETS FOR ACTIONS OF DIFFERENT LEVEL OF ZINC
O. M. Senkiv, R. Ya. Iskra, I. M. Martynyshyn, O. M. Buchko, I. Yа. Maksymovych, N. L. Tsepko
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Institute of Animal Biology NAAS,
st. Stus, 38, Lviv, 79034, Ukraine
The article shows the results of the influence of different level of zinc on the activity of enzymes of antioxidant deffence (catalase, glutathione peroxidase) in the tissues of piglets. Studies were conducted on young animals piglets, which were divided into four groups of 10–12 animals. To conduct the experiment, category of piglets aged from 28-day-old to 120-day-old respectively were selected and fed with the formula feed which is balanced in all biologically active compounds for pigs were injected with additional zinc, sulphate (ZnSO4• H2O) in paticular. The control group of animals received a diet containing 60 mg/kg zinc in the diet and the second experimental group the amount of zinc was 30 mg/kg (available in the formula feed), the second experimental group — 90 mg/kg, and the third — 120 mg/kg. The piglets were supplied with unlimited amount of formula feed and water during the experiment.
As a result of research it was established the increased activity of catalase and glutathione peroxidase (GP) in the tissues of skeletal muscle and liver in piglets II and III research groups in controlling and reducing the activity of the studied enzymes in piglets of І experimental group. However, in the process of studying the activity of enzymes in spleen tissue, we have found it was increasing in animals of І group, and it was decreasing the animals II and III experimental group. Obtained data indicate that between 28-day-old to 120-day-old daily piglets’ the optimal zinc dose on which was set a positive effect on the enzymes of antioxidant system in tissues of piglets was the amount of 90 mg/kg dose of formula feed.
Keywords: PIGLETS, ZINC, SCELETES MUSCUL, LIVER, BUDS, CATALASA, HLUTATIONPEROKSYDASА
