CATHEPSIN B MAINTENANCE IN THE LIVER OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) THROUGHOUT VIRUS INFECTION
M. I. Maistrenko, L. P. Dragan, Yu. P. Rud
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Institute of Fisheries of the National Academy of Agrarian Sciences,
Obukhivska St., 135, Kiev, 03164, Ukraine
Biochemical aspects of pathogenicity of infectious pancreatic necrosis virus (IPNV) that is the a causative agent of disease in rainbow trout (Aquabirnavirus of Birnaviridae family), in organism of rainbow trout practically not studied. Presented scientific literature data testify that for fishes, as well as for warm-blooded animals, development of many infectious diseases is accompanied by activating of proteolytic processes. Consider that destruction of lysosomes and partial exit of proteolytic enzymes in cytozole are the most early event in infected cells.In the liver of animals cells constantly there are processes of regenerations of the damaged hepatocytes, attended with activating of biochemical reactions in lysosomes.
Taking into account circumstance that there are active processes of rendering and bioaccumulation of different ksenobiotics harmless in the liver cells, including viruses, the aim of work was to study activity of cathepsin B in a liver underyearling of rainbow trout, infected by the infectious pancreatic necrosis virus.
Undertaken experimental studies showed that the infectious pancreatic necrosis virus assists activating of cathepsin B in the liver tissues of rainbow trout resulting in disintegration of proteins in the infected cells. Degradation of proteins is conditioned by strengthening of autolitic processes and lysosomal proteinases is engaged in that. One of them is a cathepsin В. Activating of cathepsin B arises up because of damage of integrity of lysosomal membrane with subsequent violation of compartmentalization of enzymes, that results in freeing and change of functioning of th lysosomal enzymes in development of return reaction of organism on the action of infectious pancreatic necrosis virus of rainbow trout.
Keywords:IPNV,CATHEPSIN B, LIVER, RAINBOW TROUT
