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BIOPHYSICAL ANALYSIS OF EMANATED PHEROMONAL ODOR CHANGES IN COWS USING ELECTRONIC NOSE TECHNOLOGY

A. Ali^1–3, F. Hussein², E. Mohamed³, H. Maher⁴

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The objective of the study was to investigate the possibility of applying the Electronic Nose (EN) technology to analyze and detect emanated pheromonal odor changes during estrus cycle in cows. In comparison to gas chromatography (GC) headspace samples analysis and blood hormonal (estradiol and progesterone) analysis using Elisa and RIA. Also to establish a protocol to appoint the proper time of artificial insemination (AI) in cows. The study was conducted on 54 Holstein-Friesian cows. Out of sensors of the EN used in this study, sensor#2 showed the highest response to all measured perineal samples, which adhered perfectly to plasma hormone (E₂) levels, and GC analysis of the perineal samples which showed progressive change in acetaldehyde as estrus was approached.

Keywords: COWS, ESTRUS, PHEROMONES, ELECTRONIC NOSE (EN), ACETALDEHYDE

1. Blasquez N. B., French J. M., Long S. E., Perry G. C. A pheromonal function for the perineal skin glands in the cow. Vet Record, 1988, vol. 123, issue 2, pp. 49–50. https://doi.org/10.1136/vr.123.2.49
2. DRG Diagnostics. Users manual: Estradiol ELISA. DRG Instrument GmbH, Germany. Division of DRG International, Inc. Available at: http://www.drg-diagnostics.de
3. DRG Diagnostics. Users manual: Progesterone ELISA. DRG Instrument GmbH, Germany. Division of DRG International, Inc. Available at: http://www.drg-diagnostics.de
4. Dulac C., Axel R. Expression of candidate pheromone receptor genes in vomeronasal neurons. Chemical Senses, 1998, vol. 23, issue 4, pp. 467–475. https://doi.org/10.1093/chemse/23.4.467
5. Klemm W. R., Rivard G. F., Clement B. A. Blood acetaldehyde fluctuates markedly during bovine estrus cycle. Animal Reproduction Science, 1994, vol. 35, issue 1–2, pp. 9–26. https://doi.org/10.1016/0378-4320(94)90003-5
6. Lane A. J. P., Wathes D. C. An electronic nose to detect changes in perineal odors associated with estrus in the cow. Journal of Dairy Science, 1998, vol. 81, issue 8, pp. 2145–2150. https://doi.org/10.3168/jds.S0022-0302(98)75791-1
7. Marchlewska-Koj A. Pheromones in mammalian reproduction. Oxford Reviews of Reproductive Biology, 1984, vol. 6, pp. 226–302.
8. Nakamoto T., Fukunda A., Moriizumi T. Perfume and flavour identification by odour-sensing system using quartz-resonator sensor array and neural-network pattern recognition. Sensors and Actuators B: Chemical, 1993, vol. 10, issue 2, pp. 85–90. https://doi.org/10.1016/0925-4005(93)80030-F
9. Nebel R. L. Components of a successful heat detection program. Advances in Dairy Technology, 2003, vol. 15, pp. 191–203. Available at: http://www.dairyweb.ca/Resources/WCDS2003/Nebel2.pdf
10. Perry G. A. Comparison of the efficiency and accuracy of three estrous detection methods to indicate ovulation in beef cattle. South Dakota Beef Report, 2005, p. 25. Available at: https://openprairie.sdstate.edu/cgi/viewcontent.cgi?referer=https://www.google.com/&httpsredir=1&article=1024&context=sd_beefreport_2005 (last accessed on April 30^th, 2019).
11. Perry G. A. Detection of standing estrus in cattle. Extension beef reproduction and management specialist animal and range sciences department, 2004. Available at: https://beefrepro.unl.edu/pdfs/heatdetection.pdf (last accessed on April 30^th, 2019).
12. Rivard G. F., Klemm W. R. Gas chromatographic analysis and estrus diagnostic potential of headspace sampling above bovine body fluids. In: Chemical Signals in Vertebrates, vol. 6, ed. by R. L. Doty and D. Müller-Schwarze. New York, Pleum Press, 1992, pp. 115–118. https://doi.org/10.1007/978-1-4757-9655-1_18

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