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Gavrylenko A, Masiuk D. The effect of organic acids mixture in the form of glycerids on the regulation of intestinal barrier function in piglets using SCFA-M: analysis of molecular markers. Bìol Tvarin. 2025; 27 (4): 30–35. DOI: 10.15407/animbiol27.04.030.
https://doi.org/10.15407/animbiol27.04.030
Received 12.07.2025 ▪ Revision 08.10.2025 ▪ Accepted 01.11.2025 ▪ Published online 16.02.2026

The effect of organic acids mixture in the form of glycerids on the regulation of intestinal barrier function in piglets using SCFA-M: analysis of molecular markers

Andrii Gavrylenko, Dmytro Masiuk
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Dnipro State Agrarian and Economic University, 25 Serhiya Yefremova str., Dnipro, 49009, Ukraine

The functions of the intestinal system are crucial for animal health and productivity, as they ensure digestion, nutrient absorption, immune homeostasis, and microbiota regulation. Molecular markers of the intestinal system, such as occludin (OCL), fibronectin (FN), interferons (IFN-α, IFN-γ), and caspase-3 (Casp-3), serve as sensitive indicators of its functional state. This article presents the results of an experimental study on the effects of short-chain fatty acids (SCFA-M, C3-C12) on the expression of molecular markers of intestinal barrier function in three-way crossbred piglets. A total of 100 piglets aged 42 days were selected for the study and divided into control and experimental groups. The diet of the experimental group was supplemented with SCFA-M. Expression levels of molecular markers were assessed in the duodenum using the Western blot method. Obtained results showed a significant increase in the expression of OCL, FN, and IFN-α in the experimental group, indicating improved barrier function, extracellular matrix stability, and immune response. Specifically, by day 56, FN expression increased by 46.80% (P<0.001), OCL by 16.78% (P<0.001), and IFN-α by 20.06% (P<0.05) compared to the control group. Contrary, decreased levels of IFN-γ and Casp-3 indicated reduced inflammation and apoptotic activity. The correlations between molecular marker expression and metabolic parameters were analysed to clarify the interaction of these indices. Notably, in 56-day-old piglets of the control group, OCL expression negatively correlated with blood total protein levels (r = −0.83; P<0.05). In 77-day-old animals of the experimental group, correlations were found between FN and OCL expression and serum calcium levels (r = −0.90; P<0.05). These findings demonstrated the positive effects of SCFA-M on immune regulation, proinflammatory balance, and the maintenance of intestinal barrier integrity in piglets. The presented data may be applied in veterinary practice for the prevention of intestinal infections, enhancement of pathogen resistance, and optimization of animal productivity.

Key words: intestinal system, molecular markers, SCFA-M, piglets, intestinal barrier function, occludin (OCL), fibronectin (FN), interferons (IFN-α, IFN-γ), caspase-3 (Casp-3)

1. Al-Sadi R, Khatib K, Guo S, Ye D, Youssef M, Ma T. Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier. Am J Physiol Gastrointest Liver Physiol. 2011; 300 (6): G1054–G1064. DOI: 10.1152/ajpgi.00055.2011.
2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72 (1–2): 248–254. DOI: 1016/0003-2697(76)90527-3.
3. Chelakkot C, Ghim J, Ryu SH. Mechanisms regulating intestinal barrier integrity and its pathological implications. Exp Mol Med. 2018; 50 (8): 1–9. DOI: 10.1038/s12276-018-0126-x.
4. Chen J, Xu YR, Kang JX, Zhao BC, Dai XY, Qiu BH, Li JL. Effects of alkaline mineral complex water supplementation on growth performance, inflammatory response, and intestinal barrier function in weaned piglets. J Anim Sci. 2022; 100 (10): skac251. DOI: 10.1093/jas/skac251.
5. Du Y, Li H, Xu W, Hu X, Wu T, Chen J. Cell surface-associated protein elongation factor Tu interacts with fibronectin mediating the adhesion of Lactobacillus plantarum HC-2 to Penaeus vannamei intestinal epithelium and inhibiting the apoptosis induced by LPS and pathogen in Caco-2 cells. Int J Biol Macromol. 2023; 224: 32–47. DOI: 10.1016/j.ijbiomac.2022.11.252.
6. Fais S, Capobianchi MR, Silvestri M, Mercuri F, Pallone F, Dianzani F. Interferon expression in Crohn’s disease patients: Increased interferon-γ and -α mRNA in the intestinal lamina propria mononuclear cells. J Interferon Res. 1994; 14 (5): 235–238. DOI: 10.1089/jir.1994.14.235.
7. Faraj AM, Ağca CA, Nedzvetsky VS, Tykhomyrov AA. C60 hydrofullerene induced autophagy and ameliorated GFAP in H₂O₂-treated human malignant glioblastoma U-373 cell line. Karbala Int J Mod Sci. 2022; 8 (3): 19. DOI: 10.33640/2405-609X.3242.
8. Gavrylenko AV, Masiuk DM. Monoglyceride supplementation modulates microbiome of small intestine in piglets. Theor Appl Vet Med. 2024; 12 (4): 21–30. DOI: 10.32819/2024.12019.
9. Groot N, Fariñas, Cabrera‑Gómez CG, Pallares FJ, Ramis G. Blend of organic acids improves gut morphology and affects inflammation response in piglets after weaning. Front Anim Sci. 2024; 5: 1308514. DOI: 10.3389/fanim.2024.1308514.
10. Kolachala VL, Bajaj R, Wang L, Yan Y, Ritzenthaler JD, Gewirtz AT, Roman J, Merlin D, Sitaraman SV. Epithelial-derived fibronectin expression, signaling, and function in intestinal inflammation. J Biol Chem. 2007; 282 (45): 32965–32973. DOI: 10.1074/jbc.M704388200.
11. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227: 680–685. DOI: 10.1038/227680a0.
12. Lan J, Chen G, Cao G, Tang J, Li Q, Zhang B, Yang C. Effects of α-glyceryl monolaurate on growth, immune function, volatile fatty acids, and gut microbiota in broiler chickens. Poult Sci. 2021; 100 (3): 100875. DOI: 10.1016/j.psj.2020.11.052.
13. Lawrance IC, Rogler G, Bamias G, Breynaert C, Florholmen J, Pellino G, Reif S, Speca S, Latella G. Cellular and molecular mediators of intestinal fibrosis. J Crohns Colitis. 2017; 11 (12): 1491–1503. DOI: 10.1016/j.crohns.2014.09.008.
14. Li Y, Guo Y, Wen Z, Jiang X, Ma X, Han X. Weaning stress perturbs gut microbiome and its metabolic profile in piglets. Sci Rep. 2018; 8 (1): 18068. DOI; 10.1038/s41598-018-33649-8.
15. Luu M, Weigand K, Wedi F, Breidenbend C, Leister H, Pautz S, Adhikary T, Visekruna A. Regulation of the effector function of CD8⁺ T cells by gut microbiota-derived metabolite butyrate. Sci Rep. 2018; 8 (1): 14430. DOI: 10.1038/s41598-018-32860-x.
16. Masiuk DM, Romanenko ER, Herrman B, Nedzvetsky VS. Fibronectin measurement as a potential molecular marker for barrier function assessment of piglet intestine. Theor Appl Vet Med. 2023; 11 (2): 3–8. DOI: 10.32819/2023.11006.
17. Moeser AJ, Pohl CS, Rajput M. Weaning stress and gastrointestinal barrier development: Implications for lifelong gut health in pigs. Anim Nutr. 2017; 3 (4): 313–321. DOI: 10.1016/j.aninu.2017.06.003.
18. Munakata K, Yamamoto M, Anjiki N, Nishiyama M, Imamura S, Iizuka S, Takashima K, Ishige A, Hioki K, Ohnishi Y, Watanabe K. Importance of the interferon-α system in large murine intestines indicated by microarray analysis of commensal bacteria-induced immunological changes. BMC Genom. 2008; 9 (1): 192. DOI: 10.1186/1471-2164-9-192.
19. Pellegrini A, Motta C, Bellan Menegussi E, Pierangelini A, Viglio S, Coppolino F, Beninati C, De Filippis V, Barbieri G, Pietrocola G. The serine-rich repeat glycoprotein Srr2 mediates Streptococcus agalactiae interaction with host fibronectin. BMC Microbiol. 2024; 24 (1): 221. DOI: 10.1186/s12866-024-03374-6.
20. Poritz LS, Sundstrom J, Harris L, Barber A, Antonetti D. 37: Alteration of occludin expression in intestinal inflammation. J Surg Res. 2009; 151 (2): 188. DOI: 10.1016/j.jss.2008.11.054.
21. Said HM. Physiology of the Gastrointestinal Tract. 6^th Academic Press, 2018. ISBN: 9780128099544. eBook ISBN: 9780128124260.
22. Talanian RV, Allen HJ. Roles of caspases in inflammation and apoptosis: Prospects as drug discovery targets. Annu Rep Med Chem. 1998; 33: 273–282. DOI: 10.1016/S0065-7743(08)61092-1.
23. Zakariah M, Molele RA, Mahdy MAA, Ibrahim MIA, McGaw LJ. Regulation of spermatogenic cell apoptosis by the pro-apoptotic proteins in the testicular tissues of mammalian and avian species. Anim Reprod Sci. 2022; 247: 107158. DOI: 10.1016/j.anireprosci.107158.