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Mushynska VS, Syrvatka VY, Shtapenko OV. Effect of long-term administration of Enterococcus sp. SB12 on oxidative stress markers of mice. Bìol Tvarin. 2026; 28 (1): 56–61. DOI: 10.15407/animbiol28.01.056.
https://doi.org/10.15407/animbiol28.01.056
Received 10.03.2026 ▪ Revision 05.05.2026 ▪ Accepted 10.05.2026 ▪ Published online 11.06.2026

Effect of long-term administration of Enterococcus sp. SB12 on oxidative stress markers of mice

V. S. Mushynska¹*, V. Y. Syrvatka^2,1, O. V. Shtapenko^3,4
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¹Іnstitute of Animal Biology NAAS, 38 V. Stusa str., Lviv, 79034, Ukraine
²Ivan Franko National University of Lviv, Genetic and Biotechnology Department, 4 Hrushevskoho St., Lviv, 79005, Ukraine.
³Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies of Lviv, Pekarska, 50, Lviv, 79010, Ukraine
⁴Lviv State University of Life Safety, Kleparivska str., Lviv, 79000, Ukraine

The study analyzes the effect of the probiotic strain Enterococcus sp. SB12 on the antioxidant defense system of mice. The relevance of the study is due to the need to search for new strains capable of modulating the redox status without depleting the internal resources of the host organism. The experiment was conducted on white laboratory mice, which were orally administered a suspension of the studied strain for 28 days. The state of the oxidative-antioxidant balance was assessed by the intensity of lipid peroxidation (content of hydroperoxides and TBA-reactive substances, the level of protein carbonyls, and catalase activity. It was found that long-term use of Enterococcus sp. SB12 does not cause significant changes in the content of lipoperoxidation products and carbonyl groups of proteins, which indicates the absence of a prooxidant effect on cellular structures. Catalase activity remained at the level of the control group, indicating a stable state of the enzymatic defense chain and the absence of metabolic stress. It was found that Enterococcus sp. SB12 does not disturb the balance of free radicals and is characterized by high metabolic safety, which makes it promising for use in probiotic preparations.

Key words: Enterococcus sp., probiotic strains, oxidative stress, catalase, TBA-reactive substances, lipid hydroperoxides, protein carbonyls, mice, oral administration

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