INFLUENCE DIAMETR STRAW AND REGIME OF THEIR THAWING ON ACTIVITY ENZYME IN PROCESS CRYOPRESERVATION BOAR SPERM
O. O. Korbetska
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Institute of Animal Biology NAAS, Lviv, 79034, st. V. Stus 38, Ukraine
In this article are given the study results of the impact of various straw diameters (2, 2.8, 5 and 7 mm) on boar spermatozoa longevity, plasma membrane and acrosome intactness after thawing. The level of spermatozoa structures damage by biochemical enzyme markers such as aspartateaminotransferase (ASТ) and acid phosphatase (AP) that leak out of the cells to the surrounded media during freezingthawing process was studied.Were used ejaculates of four Landrace boars. After ejaculate evaluation (volume, activity, concentration) semen equilibration at 5 ºC for 120 min was performed. Freezing semen in straws was performed in a volume of 0.25, 0.5, 2 and 4 ml and diameters of 2, 2.8, 5 and 7 mm, respectively, at the program cell freezer Planer R204, after which the straws were transferred to liquid nitrogen. Thawing semen was held at 37ºC for 30s, 50 ºC — 12s and 70 ºC — 8s. In plasma (medium) were measured the activity of AST and AP, also was examined longevity of spermatozoa. The lowest activity of AST and AP after thawing of porcine semen among the tested straw diameters was with the smallest diameter (2 and 2.8 mm) at thawing temperature 50 ºC for 12 s, indicating better preservation of plasma membrane integrity of spermatozoa using the combination of these straw diameters and thawing temperatures compared with other samples.
Keywords:BOAR, SPERM, CRYOPRESERVATION,STRAWS, THAWING, SURVIVAL, ASPARTATE AMINOTRANSFERASE (AST), ACID PHOSPHATASE (AP)
