THE SEX HORMONES CONTENT IN THE BLOOD AND REPRODUCTIVE ABILITI OF SOWS IMPACTED BY THE DRUG «GLUTAM 1M»WITH NEUROTROPIC AND METABOLIC ACTION
V.I. Sheremeta, L. M. Bezverhaya
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National university of life and environmental sciences of Ukraine.Str. Heroes of Defense, 15, Kiev, 43022, Ukraine
In the previous experiments,which were performed at the different times of the year, was found that feeding glutamyl 1M during artificial insemination improves the reproductive capacity of sows. The study was conducted to determine the dynamics of concentration progesterone and 17β-estradiol in the blood of sows and their reproductive ability by feeding the drug metabolic and neurotropic action.To achieve the objectives of experiment in estrus sows were selected with boars twice a day. Selected sows placed in the individual machines and dilute artificially inseminated with sperm, twice with an interval of 18 hours. There were formed a control and experimental groups with a 4 heads of sows of Large White breed. A group of females were taken one by one to identify oestrus. Formed a group of sows fatness on the principle of par-analogues on live weight (190–200 kg), age (19 months), origin (steam-chain analogs were from the same family and were inseminated with sperm of male pigs with a single line), physiological state the body (after farrowing and the second one calendar day — the same day of the sexual cycle). Sows the experimental group during the morning feeding was added to a food product «Gluten 1M» in a dose of 20 ml per 1-third day of the sexual cycle, or three times starting from the day after the first insemination. Control animals — 20 ml of normal saline.Blood for the laboratory tests were taken from the orbital sinus sow twice at 4th and 7th day of the sexual cycle (the second and fourth day after the feeding of the drug). Blood sampling was performed in the morning to feed the animals. Selected from each sow with 20 ml of blood. Serum was obtained after blood sedimentation test tube at room temperature. Formed by a blood clot in a test tube led round a thin stainless steel spokes. It was then carefully decanted into clean tubes and centrifuged for 30 minutes at 3000 rev/min. The content of hormones was determined using reagent kit firm Immunotech (Praha). The radioimmunoassay of progesterone and 17β-estradiol was carried out in a gamma counter «Gamma 800» in PE «Diagnostics Plus» (Zhitomir).
It was discovered that in the blood of sows of large white breed on day 7 of the sexual cycle progesterone concentration significantly increases almost twice compared to 4 day. In sows, which were under control, on Day 4 of the sexual cycle coefficient of correlation between the content in the blood of progesterone and 17β-estradiol was a direct high degree of r = 0.921 (p ≤ 0.95), and in research reverse moderate r = –0.735, (p ≤ 0.95). Feeding Large White sows drug «Glutam 1M» for three days starting from the day after the first insemination increases at 4, 7 day sexual cycle of blood concentration of progesterone and 17β-estradiol by 40.2, 18and 8.3 %. While in sows significantly increased fertilization, multiple and large-fruited, respectively, 11.7, 12.6 and 5.4 %.
Keywords: SOW, PROGESTERONE 17β- ESTRADIOL, A DRUG «GLUTAM 1M», FERTILITY, TWINS, MULTIPLE, CORRELATION, NEUROTROPIC AND METABOLIC EFFECTS
