PLASMA MEMBRANE AND ACROSOME INTEGRITY OF THAWED DOG SEMEN AFTER ADDITION OF SODIUM DODECYL SULFATE TO THE FREEZING EXTENDER
А. R. Korbetskyy
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Institute of Animal Biology NAAS, Lviv 79034, st. Stus 38, Ukraine
The study results of influence of different concentrations of sodium dodecyl sulfate (SDS) compared to media without any detergent and commercial OEP paste in the freezing extender of dog semen on the activity of plasma membrane and acrosome integrity of spermatozoa. As a result, it was revealed, that sperm motility immediately after thawing was highest in the SDS group compared with OER group (60.2±7.4%). The highest sperm motility among SDS groups was in a group of SDS concentration of 2 mg / ml (61.6 ±8.7%) while in the group of 4 mg/mL SDS concentration, motility was the lowest (37.0±9.3%) with a significant difference compared with other groups (p<0.05). Most of spermatozoa with intact membranes immediately after thawing was in the OEP group (64.7±12.4%). Among the SDS groups, the highest numbers were in the group with 2 mg/ml SDS concentration (63.2±9.4%) and the lowest was in a group of SDS concentration of 4 mg / ml (39.1±10.6%). The study showed no significant difference between all of SDS groups, with the exception of SDS concentration of 4 mg/ml immediately after thawing between all groups including the OEP group. Number of spermatozoa with intact acrosome was from 73.5 to 94.7 % (mean: 84.1±6.8%) before freezing. Immediately after thawing, the number of sperm with intact acrosome was 51.2±11.3% and 49.7±10.5% in groups of 2 mg/ml SDS and OEP group, respectively, values were significantly lower than before freezing (p<0.01). However, no significant difference was found between the two groups. Freezing extender for dog semen that contained 2mg/ml SDS equally shown higher effectiveness then extender suuplemented with 0.75% OEP, which was reflected in the fact that the sperm motility, plasma membrane and acrosome integrity of spermatozoa immediately after thawing not differ from the results obtained with frozen dog semen with an extender that contained OEP.
Keywords: DOGS SPERM, CRYOPRESERVATION, PLASMA MEMBRANE INTEGRITY, ACROSOME PRESERVATION, DETERGENT
