DETERMINATION OF RESIDUAL QUANTITI OF T-2 AND HT-2 TOXINS IN THE BLOOD AND ORGANS OF CHICKENS
M. V. Vovk, O. I. Fedyakova, O. M. Pazderska
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State Scientific Research Control Institute of Veterinary Drugs and Food Additives, Donetska str., 11., Lviv, 79019, Ukraine
The problem of mycotoxicosis occupied an important place in recent years because mycotoxins relevance impact on the physiological condition and efficiency of animals and birds, and also indirectly on human health. Metabolism is not always has positive effects, as a way to decrease toxicity, since, often the products are more toxic than predecessors. The goal of our study was to determinate of concentration T-2 and HT-2 toxins in blood, liver, lung and kidneys in the body of chickens.
This article describes the features of the metabolism of T-2 toxin in different species of living organisms, given the ways and means of converting individual metabolites of T-2 toxin in chickens. Theresearches conducted under the conditions of single oral administration at a dose of 0.56 mg/kg of body weight. The concentration of T-2 and HT-2 toxins in blood and organs of chickens was determined by high performance liquid chromatography using immunoaffinity columns.
The results showed that an hour after the start of the experiment, the highest concentration of toxin was detected in the blood (266.2 mg/kg). In the liver T-2 toxin is converted to HT-2, therefore the concentration of the last 6 hours after administration reaches up to 173.4 mg/kg, while in the blood, lungs and kidneys does not exceed 37.3 mg/kg. After 24 hours the content of T-2 and HT-2 toxins drastically reduces, but the kidney concentration of these compounds remains relatively high (24.21 and 86.87 mg/kg, respectively). After 48 hours in the studied samples there was only a small quantity of toxins, but the concentration of HT-2 toxin in kidneys was 27.1 mg/kg.
Keywords: T-2 TOXIN, HT-2 TOXIN, CHICKENS, HPLC, IMMUNOAFFINITY COLUMNS, METABOLISM, TRICHOTHECENE MICOTOXINS
