EFFECT OF NANOCARRIERS WITH ANTISENSE OLIGONUCLEOTIDES ON HISTOLOGICAL STRUCTURE OF ORGANS SYNTHESIZING PRION-PROTEIN

Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 91–96

http://dx.doi.org/10.15407/animbiol18.03.091

EFFECT OF NANOCARRIERS WITH ANTISENSE OLIGONUCLEOTIDES ON HISTOLOGICAL STRUCTURE OF ORGANS SYNTHESIZING PRION-PROTEIN

N. Yu. Susol, Yu. V. Martyn, N. V. Kuzmina, V. V. Vlizlo

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Institute of animal biology NAAS,
38 V.Stus str., Lviv 79034, Ukraine

Polymer compounds serves as a perspective carriers for medicinal preparations and engages antisense therapy against diseases with diverse etiology. However, the problem is in toxic effect of polymers on the animal organism. Of particular relevance acquire research on developing effective carriers of nucleotides, which could be used for prevention and treatment for disease of pathological protein synthesis.

We have been investigated the transportation and delivery of complexes of oligonucleotides with three newly polymer carriers based on dymetylaminoetylmethakrylate — MP-27, MP-2 and MP-3. The research was conducted by the example of action complexes polymer carriers with antisense oligonucleotides on cellular prion expressions. Nanocarriers influence on the structure of organs of white rats (Rattus norvegicus var. alba, Wistar line) was showed.

The complexes with polymers MP-2 and MP-3 caused the minor changes in the microstructure of the brain, small intestine and spleen. In particular, in histological structure of brain, reducing the number of neurons in sight was seen on the 7^th day after injection. In the small intestine serous membrane sprawling and Peyer plaques swelling have been detected. Histological preparations spleen revealed an increase in the size of individual follicles.

However, the effect of carrier MR-27 does not cause pathological changes in the structure of the small intestine, spleen and brain both separately and in combination with antisense oligonucleotides. So, polymeric carrier MY-27 can be used to transport of medicines.

Keywords: RATS, BRAIN, SPLEEN, INTESTINE, POLIMERIC CARRIERS, ANTISENSE OLIGONUCLEOTIDES, CELLULAR PRION

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