CONTENT AND COMPOSITION OF SOLUBLE PROTEIN IN LIVER TISSUES OF YOUNG SHEEP UNDER THE USING IN THEIR DIET ADDITIVES WITH AMINO ACIDS LYSINE, METHIONINE AND SULFATE
V. V. Havrylyak, O. S. Druzhyna, P. V. Stapay, N. P. Sydir
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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine
The effect of essential amino acids lysine, methionine and sodium sulphate as supplements to the basic diet of 4-month rams on the content and composition of the soluble protein of the liver were presented in the paper. It has been shown that when using in the diets of young rams, these supplements did not significantly affect on the total content of soluble protein in liver tissue, however their correlation was changed.
In the polyacrylamide gel the soluble proteins of liver tissue were divided into 10 fractions which were similar to some serum proteins by electrophoretic mobility. Feeding lambs with lysine and sodium sulfate in their diet (respectively 3 and 2 grams per head/day) was accompanied by significant increasing by 1,3 and 1,2 times respectively of the two fractions of proteins (2 and 4), corresponding serum γ- and β-globulins. In the animals which were fed with methionine and sodium sulphate (2 grams per head/day) there were no significant changes in the ratio of soluble proteins of liver tissue.
Using supplements with lysine, methionine and sodium sulphate in ram’s diet (respectively 3, 2 and 2 g per head/day) resulted in increased protein rate of fractions 4 and fraction 9 on 1,2 times compared with control animals. These proteins fractions correspond to serum β-globulin and albumin.
Simultaneously in the liver tissue of rams the decrease of content of fraction 3 and 6 (respectively 1,5 and 1,9 times) which correspond to serum γ- and α-globulin was observed.
It was concluded that the changes of the ratio of individual protein fractions in the liver tissue depends on lysine rather than methionine and it is related primarily to globulin.
Keywords: LAMBS, BLOOD, LYSINE, METHIONINE, SULFYR, LIVER, DIET
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