The Animal Biology

CERTAIN BIOCHEMICAL PARAMETERS AND ACTIVITY OF ANTIOXIDANT ENZYMES IN BULL BLOOD UNDER THE INFLUENCE OF CHELATE CHROMIUM COMPOUNDS

Ye. O. Dzen, I. V. Luchka, Y. T. Salyha, O. G. Malyk

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Institute of Animal Biology NAAS,
38 Stus str., Lviv 79034, Ukraine

The aim of the study was to measure certain biochemical parameters in bull blood serum and activity of antioxidant protection enzymes under feeding of 15-month-aged bull calves with Chromium (III) in Chromium methionine chelate form. Four time periods with 15 days each were applied. At the 1^st (preparatory) period, animals received the basic ration (BR), while at the 2nd, 3rd and 4th periods animals received Chromium (III) chelate compounds (0.8 mg of pure metal per day for each animal), as a solution added to the morning watering. Blood was obtained from the jugular vein 2 hours after the morning feeding, at the start of the 1st period and at the end of the 2nd, 3rd and 4th periods, and certain biochemical parameters were measured in blood serum. It is shown that the introduction of Chromium (III) in its methionine chelate form during morning feeding increased the level of urea (Р<0.05) and decreased the level of ammonia in blood at the 45th and 60th days. It also decreased (Р<0.05) glucose concentration at the 45th day from the beginning of the experiment. At the same time, Chromium supplement did not affect the content of total blood protein and the activity of superoxide dismutase. Catalase activity increased (Р<0.05) at 45th and 60^th days, while the activity of the glutathione peroxidase decreased (Р<0.05) at the 60th day of treatment. As Chromium methionine chelate caused an increase in antioxidant enzymes activity and in certain biochemical parametersof blood, we may conclude that adding organic Chromium to the feeding of bull calves is advisable.

Keywords: CATTLE, MINERALS, CHROMIUM, CHELATE, COMPOUNDS, BLOOD, SYSTEM OF ANTIOXIDANT DEFENSE

1. Snitynskyi V. V. Actual problems of feed and animal nutrition. New of agrarian sciences, 2003, no. 12, pp. 25–34. (in Ukrainian)
2. Zakharenko M. O., Shevchenko L. V., Mykhalska V. M. The role of trace elements in the life of animals. Veterinary Medicine of Ukraine, 2004, no. 2, pp. 13–16. (in Ukrainian)
3. Schermaier A. J., O’Conor L. H., Pearson K. H. Semi-automated determination of chromium in whole blood and serum by Zeeman electrothermal atomic absorption spectrophotometry. Clinical Chemistry Acta, 1985, .vol. 152, pp. 123–134. https://doi.org/10.1016/0009-8981(85)90183-4
4. Iskra R. Ja., Vlizlo V. V. The content of essential trace elements in tissues of piglets after actions of chromium chloride. The animal biology, 2012, vol. 14, no. 1–2, pp. 113–120. (in Ukrainian)
5. Solohub L. I., Antoniak H. L., Babych N. O. Chromium in humans and animals. Biochemical, immunological and environmental aspects. Lviv, Yevrosvit Publ., 2007, 128 p. (in Ukrainian)
6. Kalyn B. M. Application of chelate compounds of trace elements in animal nutrition. The methodical recommendations. Lviv, 2011, 40 p. (in Ukrainian)
7. Lowry O. H. Protein determination with the Folin phenol reagent. J. Biol. Chem., 1951, vol. 193, pp. 265–275.
8. Vlizlo V. I., Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary. A reference book. Lviv, Spolom Publ., 2012. 764 p. (in Ukrainian)
9. Kondrakhyn Y. P. Methods of veterinary clinical laboratory diagnostics: Directory. A reference book. Moscow, Kolos Publ, 2004, pp. 88–116. (in Russian)
10. Moyin V. M. Simple and specific method for determining the activity of glutathione peroxidase in erythrocytes. Laboratory work, 1986, no. 12, pp. 724–727. (in Russian)
11. Dubinina E. E., Salnikova L. A., Yefimova L. F. Activity and superoxide dismutase isozyme spectrum of red blood cells and human plasma. Laboratory work, 1983, no. 10, pp. 30–33. (in Russian)
12. Korolyuk M. A., Ivanova I. G., Mayorova I. G., Tokaryev V. E. Method of catalase activity determination. Laboratory work, 1988, no. 1, pp. 16–18. (in Russian)
13. Shigematsu S., Khan Ahmir H., Kanzaki M., Pessin J. Intracellular insulin-responsive glucose transporter (GLUT4) distribution but not insulin-stimulated GLUT4 exocytosis and recycling are microtubule dependent. Molecular endocrinology, 2002, vol. 16, no. 5, pp. 1060–1068. https://doi.org/10.1210/mend.16.5.0836
14. Halenova T. I., Raksha N. H., Savchuk O. M., Ostapenko L. I. Operation of some key enzymes of carbohydrate metabolism in rats in experimental diabetes mellitus type 2. Experimental and clinical physiology, 2011, no. 2, pp. 13–21. (in Ukrainian)
15. Yanovych V. H., Solohub L. I. Basics transformation of nutrients in ruminants. Lviv, Triada Plus Publ, 2000. p. 384. (in Ukrainian)
16. Pryor W. A. Free Radicals in Biology. Vol. 3, London, New York, San Francisco, Ed. by Academic Press, 1977, 312 p.
17. Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans., 2007, vol. 35, no. 5, pp. 1147–50. https://doi.org/10.1042/BST0351147

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