Influence of liposomal drug on the intensity of proteins oxide modification processes in subclinical mastitis of cows

V. A. Chepurna¹, T. M. Suprovych¹, O. I. Vishchur², V. P. Mizik¹, I. Ye. Solovodzinska³

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¹Podillia State University,
13 Shevchenko str., Kamianets-Podilskyi, Khmelnytsk region, 32316, Ukraine

²Institute of Animal Biology NAAS,
38 V. Stus str., Lviv, 79034, Ukraine

³Lviv National Agrarian University,
1 Volodymyr Velykyi str., Dubliany, Zhovkva district, Lviv region, 80381, Ukraine

The article contains the experimental studies of the liposomal drug based on plant raw materials ― hypericum (Hypericum perforatum L.) effect on the intensity of oxidative modification of proteins (OMP) in the blood and milk of cows with subclinical mastitis. Studies have shown that cows with signs of subclinical form of mastitis in the serum have an increase in the content of aldehyde-derived OMP₃₇₀ and ketone-derived OMP₄₃₀, respectively, 1.3 and 1.2 times relative to similar indicators in healthy animals. In the milk of sick cows, the content of derivatives OMP₃₇₀ and OMP₄₃₀ was1.99 and 2.29 times higher, respectively, than in animals of the control group. At the beginning of the study sick cows’ milk was recorded a significantly low value of the activity of the enzymatic link of antioxidant protection ― superoxide dismutase. At the same time, a 2.6-fold (P<0.001) increase in the number of somatic cells was noted compared to their number in the milk of clinically healthy cows. Intracisternal injection of liposomal drug to cows caused a decrease in the intensity of oxidative processes. In the blood of sick cows the content of aldehyde derivatives OMP₃₇₀ on the 9^th day of the experiment was 23.1% (P<0.05) less than before the drug, and in milk the content of OMP₃₇₀ decreased by 61.8% (P<0.01). Similar changes were observed with respect to the level of ketone derivatives. In particular, on the 9th day of the experiment, the content of OMP₄₃₀ decreased by 11.7% (P<0.05) compared with its value in the blood of sick animals before the introduction of the study drug, and in milk it decreased by 64.2% (P<0.01). During the treatment on the 9^th day of the experiment, the number of somatic cells in milk decreased by 41.8% (P<0.01). In the course of treatment on the 3^rd and 9^th day there was a tendency to increase superoxide dismutase activity in the milk of sick cows compared with the beginning of the experiment. Thus, intracisternal injection of liposomal drug to cows with subclinical mastitis leads to a decrease in aldehyde and ketone derivatives of proteins oxidative modification in serum and milk. At the same time, an increase in the activity of the enzymatic link of antioxidant protection and a decrease in the number of somatic cells in the milk of cows were recorded.

Key words: cows, subclinical mastitis, somatic cells, oxidative modification of proteins, liposomal drug

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