MOLECULAR GENETIC ANALYSIS BOAR BREEDS MAJOR WHITE AND LANDRACE GENES BY ESTROGEN AND PROLACTIN RECEPTORS
V. I. Sheremeta, A. S. Opanasenko
This email address is being protected from spambots. You need JavaScript enabled to view it.
National University of Life and Environmental Sciences of Ukraine, Heroes of Defense Str., 15, Kiev–43022, Ukraine
The studies were conducted to assess the genetic potential of breeding boars breeds Landrace and Large White on the genes of estrogen receptor (ESR) and prolactin (PRLR), establishing the level and differences in sperm-productivity in various media of genotypes of these genes.
Genomic DNA was gathered from blood using reagents supplied in the «DNA-sorb B» (AmpliSens, Russia).The Analysis of the genotypes of pigs were conducted by PCR-RFLP (polymerase chain reaction, length’s polymorphism of the restriction fragments). In the obtained product, after amplification of genes ESR and PRLR at 37 °C, was plugged in restriction enzyme Pvu II and Alu I respectively and aged for 12–14 hours. Received restriction fragments were separated in a 4% agarose gel (Helicon, Russia). There were obtained fragments, for the genotype by the ESR gene: AA — 120 bp., BB — 65 bp. and 55 bp., AB — 120bp., 65 bp. and 55 bp. PRLR genotypes in gene fragments corresponded to the following: AA — 85 bp., 59 bp., 19 bp., BB —- 104 bp., 59 bp., AB — 104 bp., 85 bp., 59 bp., 19 bp.
It was found that in the analyzed sample of boars Landrace and Large White there is the quantitative differences in the genotypes of the genes PRLR and ESR .Thus, all studied Landrace boars and most (60%) of large white were heterozygous genotype AB gene ESR. Whereas, in prolactin receptor gene observed single heterozygous individuals, and most maker of both breeds are homozygous have genotype BB. Breed differences relates to the fact that the breed Landrace boars had only heterozygous genotype for the gene ESR, and the breed «Large white» have 40% of the animals with the AA genotype. In Landrace breed frequency of allele A in gene PRLR is higher up to 12% compared to the «Large white» breed. Moreover, Landrace boars with different genotypes outscored sperm-productivity manufacturers of large white breed. Within each species, not found benefits of boars sperm-productivity one of the genotypes of the genes and receptors PRLR ESR.
Key words: BOARS, GENE PROLACTIN RECЕPTOR, GENE ESTROGEN RECЕPTOR, GENOTYPE FREQUENCIES, ALLELE, CONCENTRATION,SPERM, EYACULATE, QUANTITY SPERMS, BLOOD
