SOME LIPID CLASSES CONTENT IN BROILER CHICKENS BLOOD AT PRE-SLAUGHTER STRESS
S.S. Grabovskyi
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Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Lviv, 79010, Pekarska st., 50, Ukraine
The results of researches of some lipid classes content (phospholipids, monoacylglicerols, diacylglicerols, triacylglicerols, non-etherified fatty acids (NEFA), cholesterol and its etherified form) in broiler chickens blood plasma before slaughter are presented in the article. The spleen extract (biologically active substances) has been additionally entered to the basic ration of experimental groups of bird by aerosol method which was obtained with and without application an ultrasound. The content of such phospholipids as lysolecithin, sphingomielin, phosphatidylserine, phosphatidylcholine, phosphatidylinositole, phosphatidylethanolamine, cardiolipin, phosphatidic acid in broiler chickens blood was determined.
The results about influence biologically active compounds from the spleen extract at for pre-slaughter stress on separate lipids and phospholipids classes content in broiler chickens blood plasma were obtained.
Before slaughter the phospholipids are prevailed in broiler chickens blood plasma of all experimental groups, less content of free cholesterol and triglycerides content was observed.
The highest level of phospholipids and NEFA content was observed before slaughter crucial period in broiler chickens blood of the first experimental group, which additionally to the basic ration entered the spleen extract, obtained with the application of ultrasound. Such fractions of phosphotides prevail in broiler chickens blood plasma of all groups: lysolecithin and phosphatidylethanolamine, while less than there is phosphatidylcholine, phosphatidylinositole and phosphatidic acid.
The lysolecithine level rose in broiler chickens blood plasma of the first experimental group, and phosphatidic acid — reduced almost in three times; phosphatidylcholine content diminished a little. The phosphatidylinozitole level reduced, and phosphatidylethanolamine — rose almost twice in broiler bhickens blood plasma of all experimental groups.
Keywords: PRE-SLAUGHTER STRESS, SPLEEN EXTRACT, BROILER CHICKENS, BLOOD PLASMA, LIPID CLASSES