TO THE METHOD OF OBTAINING OF BONE MARROW IN DOGS
A. Y. Mazurkevych, М. О. Malyuk, S. M. Tkachenko, I. O. Kharkevych
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National University of Life and Environmental Sciences of Ukraine,
Heroiv Oborony st., 15; Kyiv, 03041, Ukraine
In selecting a bone for obtaining of bone marrow is paying attention not only size and location of bone, but also the correspondence in structure of spongy and compact tissue. In the list of bone, suitable for obtaining of bone marrow in animals, due to presence predominantly of cancellous bone tissue rich in stem elements, are primarily sternum and iliac bone. As are known, bones, besides the compact tissue, in the epiphyses area are built of cancellous tissue, which is their foundation and is also a source of stem cells.
The results of modification the method of obtaining of bone marrow from cancellous bones in small animals (for example, dogs) are presented.
In selecting a bone for obtaining of bone marrow and development of methods of obtaining it primarily guided by ability of surgical access and surgery performance, speed of implementation, presence of cancellous tissue, expected volume of bone marrow aspirate, necessity of post-operative care of animals and probability of occurrence of different sorts of complications.
Established that choice of bone marrow (bone type, place of puncture) and method of obtaining affects on the convenience of manipulations, speed of their implementation, degree of soft tissues injury, need for suture material using and postoperative care of the animals. The method of obtaining of bone marrow in the area of proximal epiphysis of humerus and distal epiphysis of femoral bones is affordable and simple in execution, provides sufficient volume of aspirates.
Keywords: BONE MARROW, BONE TISSUE, STEM CELLS, METHOD, ACCESS
