PEROXIDATION PROCESSES IN TISSUES AND REPRODUCTIVE ABILITY OF CARPS-SIRES WITH DIFFERENT LEVELS OF VITAMIN A IN FODDER

Bìol. Tvarin, 2016, vol. 18, no. 1, pp. 93–98

https://doi.org/10.15407/animbiol18.01.093

PEROXIDATION PROCESSES IN TISSUES AND REPRODUCTIVE ABILITY OF CARPS-SIRES WITH DIFFERENT LEVELS OF VITAMIN A IN FODDER

M. Maletich

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Institute of agriculture in the Carpathian region NAAS,
5 Grushevskyi str., Obroshino village, Pustomyty district, Lviv region, 81115, Ukraine

Breeding of commodity fish and increasing its productivity are important problems in fish farming. Preservation of product units, rapid weight gain, total release of larvae, high resistance to diseases are the actual problems of fish farming in Ukraine due to a number of etiological factors which lead to disruption of growth and metabolism in fish body. These disorders can be caused by lack of vitamins, particularly vitamin A.

The aim of this research was to investigate the effect of increased amount of vitamin A in the diet on the activity of antioxidant enzymes and the content of the products of lipid peroxidation in tissues and the reproductive ability of carps-sires. Research was conducted in the pre-spawning period in three groups of carps-sires. The carps of control group received standard pelleted feed. The carps-sires of research group received retinylacetate in the composition of the above-mentioned feed.

It was found that in skeletal muscle of female and male carps-sires of the experimental group which in the pre-spawning period received vitamin A in the amount of 2500 and 5000 IU/kg diet as part of standard granulated feed the activity of the main enzymes of antioxidant protection (superoxide dismutase, glutathione peroxidase and catalase) increased significantly and dose-dependently. Under these conditions the level of diene conjugates and malonic dialdehyde in the liver of female carp-sires and the level of diene conjugates hydroperoxides lipids and malonic dialdehyde in the skeletal muscle of female carp-sires and in liver and skeletal muscles of male carp-sires reduced dose-dependently and significantly. In the pre-spawning period in carps of the experimental groups which were fed additionally with vitamin A in the amount of 2500 and 5000 IU/kg of fodder as part of standard granulated feed the working and relative fecundity in female carps-sires and the volume of milts in males increased significantly and dose-dependently. The release of larvae from eggs increases too.

Keywords: CARPS-SIRES, ENZYMS, LIPIDS, PEROXIDATION PROCESSES, REPRODUCTIVE ABILITY, FECUNDITY, RETINYLACETATE

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